Cryptosporidium molnari n. sp (Apicomplexa: Cryptosporidiidae) infecting two marine fish species, Sparus aurata L. and Dicentrarchus labrax L.
Cryptosporidium molnari n. sp (Apicomplexa: Cryptosporidiidae) infecting two marine fish species, Sparus aurata L. and Dicentrarchus labrax L.
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DOI：
10.1016/s0020-7519(02)00058-9
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发表时间：
2002-07-01
影响因子：
4
通讯作者：
Sitjà-Bobadilla, A
中科院分区：
文献类型：
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作者：
Alvarez-Pellitero, P;Sitjà-Bobadilla, A
莫尔纳隐孢子虫sp.的描述，从两个硬骨鱼，金头鲷（Sparus aurata L.）和欧洲鲈鱼（Dicentrarchus labrax L.）。寄生虫主要分布于胃上皮，肠道内少见。卵囊几乎是球形的，有四个裸露的子孢子和一个突出的残留物，并测量3.23-5.45 × 3.02-5.04（平均4.72 × 4.47）妈妈的模式宿主，金头鲷（形状指数1-1.17，平均1.05）。孢子形成是内源性的，因为在鱼体内发现了完全孢子化的卵囊，无论是在胃上皮和管腔中，还是在粪便中。卵囊和C. Molnari符合隐孢子虫属的大多数特征，但与迄今为止描述的物种，包括鱼类的物种不同。所有阶段都位于一个由双宿主微绒毛膜衬里的宿主贡献的寄生虫液泡内。裂殖体和配子体阶段出现在典型的细胞质外的位置，而卵殖体和孢子生殖体阶段位于上皮内深处。超微结构特征，包括寄生虫与宿主上皮表面的特征性接触带，大多与其他隐孢子虫属一致。线粒体存在于裂殖体、裂殖子、小配子体和子孢子中。在金头海鲷中，病理学影响更为明显，这也表现出明显更高的患病率（24.4%对4.64%）。与重要的组织病理学损伤相比，很少观察到外部临床体征，包括白色粪便、腹部肿胀和腹水。被卵原细胞和孢子生殖细胞阶段侵入的上皮的宽区域出现坏死，具有丰富的细胞碎片和脱落的上皮细胞，其脱离到管腔。未观察到炎症反应，细胞反应仅限于参与吞噬上皮内阶段和碎片的细胞，可能是巨噬细胞。(C)2002年澳大利亚寄生虫学会由Elsevier Science Ltd.出版，版权所有。
Cryptosporidium molnari n. sp. is described from two teleost fish, the gilthead sea bream (Sparus aurata L.) and the European sea bass (Dicentrarchus labrax L.). The parasite was found mainly in the stomach epithelium and seldom in the intestine. Oocysts were almost spherical, with four naked sporozoites and a prominent residuum, and measured 3.23-5.45 x 3.02-5.04 (mean 4.72 x 4.47) mum in the type host, gilthead sea bream (shape index 1-1.17, mean 1.05). Sporulation was endogenous, as fully sporulated oocysts were found within the fish, both in the stomach epithelium and lumen, and in faeces. Oocysts and other stages of C. molnari fit most of the diagnostic features of the genus Cryptosporidium, but differ from hitherto described species, including piscine ones. All stages were located within a host contributed parasitophorous vacuole lined by a double host microvillar membrane. Merogonial and gamogonial stages appeared in the typical extra-cytoplasmic position, whereas oogonial and sporogonial stages were located deeply within the epithelium. Ultrastructural features, including the characteristic contact zone of the parasite with the host epithelial surface, were mostly coincident with those of other Cryptosporidium spp. Mitochondria were found in dividing meronts, merozoites, microgamonts and sporozoites. Pathological effects were more evident in gilthead sea bream, which also exhibited a clearly higher prevalence (24.4 versus 4.64% in sea bass). External clinical signs, consisting of whitish faeces, abdominal swelling and ascites, were rarely observed, in contrast with important histopathological damage. The wide zones of epithelium invaded by oogonial and sporogonial stages appeared necrotic, with abundant cell debris, and sloughing of epithelial cells, which detached to the lumen. No inflammation reaction was observed and the cellular reaction was limited to the cells involved in the engulfing of intraepithelial stages and debris, probably macrophages. (C) 2002 Australian Society for Parasitology Inc. Published by Elsevier Science Ltd. All rights reserved.