Chronic cervical spinal cord injury and autonomic hyperreflexia in rats.
Chronic cervical spinal cord injury and autonomic hyperreflexia in rats.
复制标题
大鼠慢性颈脊髓损伤和自主神经反射亢进。
DOI：
10.1152/ajpregu.1990.258.1.r169
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Schramm,LP
中科院分区：
文献类型：
--
作者：
Osborn,JW;Taylor,RF;Schramm,LP
尽管众所周知，颈脊髓损伤患者容易出现急性、明显的高血压发作，即自主神经反射亢进，但介导这种有时致命的现象的具体机制尚不完全清楚。在本报告中，我们描述了慢性颈脊髓损伤和自主神经反射亢进大鼠模型的制备和表征。成年雄性斯普拉格-道利大鼠长期装有动脉、静脉和胃导管。从完全颈椎横断术 (CST) 后的第一天开始并持续 1 周，研究了急性高血压对膀胱压力适度增加 (0-20 mmHg) 的反应。 CST 后第一天膀胱扩张期间平均动脉压增加 25.9 +/- 4.8 mmHg。 CST 后 3、5 和 7 天的这种反应没有显着差异（总体平均值 = 18.0 +/- 2.3 mmHg）。膀胱扩张的升压反应被膀胱内注射利多卡因和自主神经节阻断（阿托品+六甲铵）完全消除。施用氯醛糖麻醉后未观察到对膀胱扩张的反应。我们的结论是，颈椎横断后，大鼠表现出与脊髓损伤的人类相似的自主神经反射亢进。此外，大鼠CST后24小时内完全建立自主神经反射亢进。最后，大鼠的一些脊髓自主反射被氯醛糖麻醉所抑制。
Although it is well established that patients with cervical spinal cord injury are prone to acute, marked, hypertensive episodes, i.e., autonomic hyperreflexia, the specific mechanisms mediating this sometimes-fatal phenomenon are not completely understood. In this report, we describe the preparation and characterization of a rat model of chronic cervical spinal cord injury and autonomic hyperreflexia. Adult male Sprague-Dawley rats were chronically instrumented with arterial, venous, and gastric catheters. Beginning the first day after a complete cervical spinal transection (CST) and continuing for 1 wk, acute hypertensive responses to a modest increase of urinary bladder pressure (0-20 mmHg) were studied. Mean arterial pressure increased 25.9 +/- 4.8 mmHg during bladder distension the first day after CST. This response was not significantly different 3, 5, and 7 days after CST (overall average = 18.0 +/- 2.3 mmHg). The pressor response to bladder distension was completely abolished by intravesical lidocaine and autonomic ganglionic blockade (atropine + hexamethonium). Responses to bladder distension were not observed after the administration of chloralose anesthesia. We conclude that after cervical spinal transection the rat exhibits autonomic hyperreflexia similar to that seen in humans with spinal injury. Furthermore, autonomic hyperreflexia is completely established within 24 h after CST in the rat. Finally, some spinal autonomic reflexes are suppressed by chloralose anesthesia in the rat.