Significance and evaluation of anaplastic lymphoma kinase by immunohistochemistry in non-small cell lung cancer
Significance and evaluation of anaplastic lymphoma kinase by immunohistochemistry in non-small cell lung cancer
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免疫组化检测间变性淋巴瘤激酶在非小细胞肺癌中的意义及评价
DOI：
10.1007/s13277-016-4946-9
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发表时间：
2016-02
期刊：
影响因子：
--
通讯作者：
Wang, Enhua
中科院分区：
文献类型：
--
作者：
Zhao, Huanyu;Jiang, Guiyang;Zhang, Xiupeng;Wang, Enhua
我们使用免疫组织化学和逆转录聚合酶链反应（RT-PCR）分别评估283例野生型表皮生长因子受体（EGFR）非小细胞肺癌活检标本中间变性淋巴瘤激酶（ALK）蛋白表达和基因重排。 52 例 (18.4%) 免疫组织化学呈 ALK 阳性，各种单克隆抗体（罗氏 ALK 检测试剂盒、D5F3、p-ALK 和 EML4-ALK）染色无显着差异。 RT-PCR 发现 36 例 (12.7%) 呈 ALK 阳性。免疫组化和RT-PCR均阳性35例，均阴性230例，一致性较高（265/283，93.6%）。其中免疫组化阳性17例，RT-PCR阴性，1例免疫组化阴性，RT-PCR阳性。对这18例进行荧光原位杂交（FISH）检测，仅3例呈阳性（1例RT-PCR阳性；2例免疫组化阳性）。野生型 EGFR 非小细胞肺癌中 ALK 阳性的患病率很高。免疫组织化学检测 ALK 基因重排与 RT-PCR 高度一致，因此是一种很好的筛查工具，但会产生假阳性结果，需要通过 RT-PCR 或 FISH 进一步筛查。
We used immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR) to evaluate anaplastic lymphoma kinase (ALK) protein expression and gene rearrangements, respectively, in 283 cases of wild-type epidermal growth factor receptor (EGFR) non-small cell lung cancer biopsy specimens. Immunohistochemistry was positive for ALK in 52 cases (18.4 %), and there was no significant difference in staining between various monoclonal antibodies (Roche ALK test kit, D5F3, p-ALK, and EML4-ALK). On RT-PCR, 36 cases (12.7 %) were positive for ALK. Immunohistochemistry and RT-PCR were both positive in 35 cases and both negative in 230 cases, and both have a high consistency (265/283, 93.6 %). Including 17 cases, immunohistochemistry was positive but RT-PCR was negative, and in one case, immunohistochemistry was negative but RT-PCR was positive. On fluorescence in situ hybridization (FISH) testing of these 18 cases, only three cases were positive (one RT-PCR was positive; two immunohistochemistry were positive). There is a high prevalence of ALK positivity in wild-type EGFR non-small cell lung cancer. Immunohistochemistry for the detection of ALK gene rearrangements was highly consistent with RT-PCR, and thus, it is a good screening tool but produces false positive results that necessitate further screening by RT-PCR or FISH.
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影响因子：
3.7
作者：
Li Y;Pan Y;Wang R;Sun Y;Hu H;Shen X;Lu Y;Shen L;Zhu X;Chen H
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Chen H
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20.4
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作者：
Hutarew, Georg;Hauser-Kronberger, Cornelia;Dietze, Otto
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4.1
作者：
Minca, Eugen C.;Portier, Bryce P.;Tubbs, Raymond R.
通讯作者：
Tubbs, Raymond R.