Identification of immunodominant linear epitopes from SARS-CoV-2 patient plasma
Identification of immunodominant linear epitopes from SARS-CoV-2 patient plasma
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DOI：
10.1371/journal.pone.0238089
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发表时间：
2020-09-09
期刊：
影响因子：
3.7
通讯作者：
Winssinger, Nicolas
中科院分区：
文献类型：
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作者：
Farrera-Soler, Lluc;Daguer, Jean-Pierre;Winssinger, Nicolas
新型严重急性呼吸综合征冠状病毒（SARS-CoV-2）是当前COVID-19大流行的源头，对公共卫生和经济稳定造成毁灭性后果。利用肽阵列来绘制愈合患者（12）和健康患者(6)血浆的抗体反应，我们确定了三个免疫优势线性表位，其中两个对应于已知对细胞进入至关重要的刺突蛋白（S1/S2和S2’）上的关键蛋白水解位点。我们展示了生化证据，S1/S2切割位点附近表位的血浆阳性抑制了furin介导的刺突蛋白水解。
A novel severe acute respiratory syndrome coronavirus (SARS-CoV-2) is the source of a current pandemic (COVID-19) with devastating consequences in public health and economic stability. Using a peptide array to map the antibody response of plasma from healing patients (12) and heathy patients (6), we identified three immunodominant linear epitopes, two of which correspond to key proteolytic sites on the spike protein (S1/S2 and S2') known to be critical for cellular entry. We show biochemical evidence that plasma positive for the epitope adjacent to the S1/S2 cleavage site inhibits furin-mediated proteolysis of spike.