Isolation and characterization of Kingella negevensis sp nov., a novel Kingella species detected in a healthy paediatric population
Isolation and characterization of Kingella negevensis sp nov., a novel Kingella species detected in a healthy paediatric population
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DOI：
10.1099/ijsem.0.001957
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发表时间：
2017-07-01
影响因子：
2.8
通讯作者：
Fournier, Pierre-Edouard
中科院分区：
文献类型：
--
作者：
El Houmami, Nawal;Bakour, Sofiane;Fournier, Pierre-Edouard
我们在此报告了从以色列和瑞士健康儿童口咽部培养的21株革兰氏阴性菌株的分离和特征。最初描述为小菌落变种的Kingella kingae，表型分析，生化分析，基于部分16 S rRNA基因和5个管家基因（abcZ，adk，G6 PD，groEL和recA）的测序的系统发育分析，以及全基因组测序和比较Kingella属和奈瑟氏菌属的成员提供了将它们分配到Kingella属的证据。细胞脂肪酸包括大量的C-12：0、C-14：0、C-16：0和C-16：1 n7。Sch 538（T）和K. kingae ATCC 23330（T）的相关性为19.9%。比较分析GenBank中的16 S rRNA基因序列，可以与美国分离的菌株相匹配，表明其地理分布更广。由于大多数菌株都是在以色列南部沙漠地区内盖夫分离到的，因此提出了一种名为Kingella negevensis sp. nov.的新物种。模式菌株为Sch 538（T）（=CCUG 69806（T）=CSUR P957）。
We herein report the isolation and characterization of 21 Gram- stain- negative strains cultivated from the oropharynx of healthy children in Israel and Switzerland. Initially described as small colony variants of Kingella kingae, phenotypic analysis, biochemical analysis, phylogenetic analysis based on sequencing of the partial 16S rRNA gene and five housekeeping genes (abcZ, adk, G6PD, groEL and recA), and whole genome sequencing and comparison between members of the genera Kingella and Neisseria provided evidence for assigning them to the genus Kingella. Cellular fatty acids included important amounts of C-12 : 0, C-14 : 0, C-16 : 0 and C-16 : 1n7. Digital DNA- DNA hybridization between the isolates Sch538(T) and K. kingae ATCC 23330(T) revealed relatedness of 19.9 %. Comparative analysis of 16S rRNA gene sequences available in GenBank allowed matches to strains isolated in the USA, suggesting a wider geographical distribution. A novel species named Kingella negevensis sp. nov. is proposed, as most strains have been isolated in the Negev, a desert region of southern Israel. The type strain is Sch538(T) (=CCUG 69806(T)=CSUR P957).