Iron-Deficiency Stress Responses in Cucumber (Cucumis sativus L.) Roots (A Possible Role for Ethylene?)
Iron-Deficiency Stress Responses in Cucumber (Cucumis sativus L.) Roots (A Possible Role for Ethylene?)
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DOI：
10.1104/pp.105.4.1133
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发表时间：
1994-08
期刊：
影响因子：
--
通讯作者：
F. Romera;E. Alcántara
中科院分区：
文献类型：
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作者：
F. Romera;E. Alcántara
大多数双子叶植物对缺铁的反应是通过几种被称为缺铁胁迫反应的机制来实现的。为了研究这些反应的调控，黄瓜幼苗（Cucumis sativus L。cv阿什利）在营养液中培养11 d，最后4 d或5 d缺铁。乙烯合成抑制剂（2或10 [mu]M氨基乙氧基乙烯基甘氨酸; 10或20 [mu]M氨基氧基乙酸; 1、2、5或10 [mu]M Co2+，如CoCl 2）或作用抑制剂（50、200或800 [mu]M Ag+，如硫代硫酸银）在此生长期间的不同时间加入到营养液中，不含Fe。在此之后，通过阅读2小时后在562 nm处的吸光度，用亚铁嗪测量整个植物的根对Fe 3+乙二胺四乙酸盐的还原。营养液中乙烯抑制剂的存在抑制缺铁胁迫反应，铁还原能力和根尖下根肿胀。在另一个实验中，向具有低铁还原活性的植物的营养液中添加1 [mu]M 1-氨基环丙烷-1-羧酸（ACC）（乙烯合成的前体）显著增加了铁还原能力和近根尖根膨胀。在这里，我们表明，乙烯在缺铁胁迫反应的发展中发挥了作用，因为当乙烯的合成或作用被抑制，反应也被抑制，当乙烯的前体（ACC）被添加，反应增加。
Most dicotyledonous species respond to Fe deficiency by developing several mechanisms known as Fe-deficiency stress responses. To study the regulation of these responses, young cucumber plants (Cucumis sativus L. cv Ashley) were grown in nutrient solution for 11 d, being deprived of Fe during the last 4 or 5 d. Inhibitors of ethylene synthesis (2 or 10 [mu]M aminoethoxyvinylglycine; 10 or 20 [mu]M aminooxyacetic acid; 1, 2, 5, or 10 [mu]M Co2+ as CoCl2) or action (50, 200, or 800 [mu]M Ag+ as silver thiosulfate) were added to the nutrient solution at different times during this period of growth with no Fe. After this period, the reduction of Fe3+ ethylenedi-aminetetraacetate by the roots of entire plants was measured with ferrozine by reading the absorbance at 562 nm after 2 h. The presence of the ethylene inhibitors in the nutrient solution inhibited the Fe-deficiency stress responses ferric-reducing capacity and subapical root swelling. In another experiment, the addition of 1 [mu]M 1-aminocyclopropane-1-carboxylic acid (ACC), a precursor of ethylene synthesis, to the nutrient solution of plants having low ferric-reducing activity increased notably the ferric-reducing capacity and subapical root swelling. Here we show evidence that ethylene plays a role in the development of Fe-deficiency stress responses, since when ethylene synthesis or action was inhibited, the responses were also inhibited, and when a precursor of ethylene (ACC) was added, the responses were increased.