Regulatory Effects of Stachyose on Colonic and Hepatic Inflammation, Gut Microbiota Dysbiosis, and Peripheral CD4+ T Cell Distribution Abnormality in High-Fat Diet-Fed Mice
Regulatory Effects of Stachyose on Colonic and Hepatic Inflammation, Gut Microbiota Dysbiosis, and Peripheral CD4+ T Cell Distribution Abnormality in High-Fat Diet-Fed Mice
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水苏糖对高脂饮食小鼠结肠和肝脏炎症、肠道菌群失调和外周 CD4 T 细胞分布异常的调节作用
DOI：
10.1021/acs.jafc.9b04731
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发表时间：
2019-10-23
影响因子：
6.1
通讯作者：
Yang, Xingbin
中科院分区：
文献类型：
--
作者：
Liu, Yueyue;Li, Ting;Yang, Xingbin
长期的高脂肪饮食(HFD)会导致一系列健康问题。肠道微生物区系在HFD相关炎症的发生发展中起着决定性的作用，参与T细胞的功能。本研究旨在探讨膳食水苏糖对高脂饮食诱导的小鼠体重增加、炎症反应、肠道微生物区系失调和T细胞异常的调节作用。将小鼠分为正常饲料组、高脂饲料组和高脂饲料加水苏糖(400 mg/kg)组。结果表明，水苏糖可减轻HFD引起的小鼠结肠和肝脏炎症，减轻HFD引起的小鼠血清肿瘤坏死因子-α水平的升高和外周血白细胞数的升高。肠道微生物区系分析表明，水苏糖通过提高肠道细菌的多样性，改善了高脂饲料喂养小鼠肠道的动态平衡，提高了肠道细菌的相对丰度，增加了普氏杆菌NK3B31_群、副紫锥菌、克里斯滕斯纳科_R-7_群和厌氧杆菌的相对丰度，并降低了粪便中丁酸的含量，同时降低了纤毛菌科_N-K4A136_群、硫磺弧菌、厌氧干菌群、粘螺杆菌、玫瑰花杆菌和Odoriter的丰度。流式细胞仪分析显示水苏糖能拮抗HFD引起的小鼠外周血中CD4(+)T细胞数量的减少。综上所述，这些发现提示长期服用水苏糖可以通过调节肠道微生物区系来改善HFD相关的结肠和肝脏炎症及其并发症。
A long-term high-fat diet (HFD) can cause a range of health problems. Gut microbiota plays a decisive role in the development of HFD-associated inflammation, involved in function of T cells. This study was designed to probe the regulative effects of dietary stachyose, a functional oligosaccharide, on HFD-induced weight gain, inflammation, gut microbiota dysbiosis, and T cell abnormality in C57Bl/6 mice. Mice were divided into three groups which received normal chow, HFD and HFD plus stachyose (400 mg/kg), respectively. Results showed that administration of stachyose diminished the HFD-induced upregulation of serum TNF-alpha level and elevation of peripheral blood leukocyte populations to alleviate the HFD-caused colonic and hepatic inflammation in mice. Analysis of gut microbiota revealed that stachyose improved the intestinal homeostasis of HFD-fed mice by improving the bacterial diversity with the increases in the relative abundances of the Prevotellaceae_NK3B31_group, Parasutterella, Christensenellaceae _R-7_group, and Anaerovorax, as well as the fecal level of butanoic acid, while decreasing the ratio of Firmicutes-to-Bacteroidetes and the abundances of the Lachnospiraceae_N-K4A136_group, Desulfovibrio, Anaerotruncus, Mucispirillum, Roseburia, and Odoribacter. Flow cytometric analysis showed that stachyose antagonized the HFD-induced decrease of peripheral CD4(+) T cell population in mice. Conclusively, these findings suggest that long-term consumption of stachyose can ameliorate the HFD-associated colonic and hepatic inflammation and its complications by modulating gut microbiota.