Development of a Cyclic Strain Bioreactor for Mechanical Enhancement and Assessment of Bioengineered Myocardial Constructs.
Development of a Cyclic Strain Bioreactor for Mechanical Enhancement and Assessment of Bioengineered Myocardial Constructs.
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DOI：
10.1007/s13239-015-0236-8
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发表时间：
2015-12
影响因子：
1.8
通讯作者：
Birla RK
中科院分区：
文献类型：
--
作者：
Salazar BH;Cashion AT;Dennis RG;Birla RK
本研究的目的是开发可行的生物反应器技术，使用一种新的音圈致动器系统来研究周期性应变对大鼠心肌细胞制造的心脏贴片的影响。本研究中使用的生物工程肌肉结构是通过在纤维蛋白凝胶上培养大鼠新生心肌细胞形成的。生物反应器的物理设计最初是使用Solidworks来测试间隙并进行结构应变分析。一旦软件设计阶段完成，生物反应器就会使用商用、定制机械和3d打印部件的组合进行组装。我们利用生物反应器评估4小时拉伸方案对组织收缩特性的影响，之后对组织进行免疫组织学评估。在1Hz拉伸10%的应变方案后，观察到收缩力增加，而对照组无明显增加。此外，心肌肌原纤维排列、连接蛋白43表达和I型胶原分布均有所增加。在这项研究中，我们证明了一种新的生物反应器设计的有效性，以提高工程心肌组织的收缩性。
The purpose of this study was to develop enabling bioreactor technologies using a novel voice coil actuator system for investigating the effects of periodic strain on cardiac patches fabricated with rat cardiomyocytes. The bioengineered muscle constructs used in this study were formed by culturing rat neonatal primary cardiac cells on a fibrin gel. The physical design of the bioreactor was initially conceived using Solidworks to test clearances and perform structural strain analysis. Once the software design phase was completed the bioreactor was assembled using a combination of commercially available, custom machined, and 3-D printed parts. We utilized the bioreactor to evaluate the effect of a 4-hour stretch protocol on the contractile properties of the tissue after which immunohistological assessment of the tissue was also performed. An increase in contractile force was observed after the strain protocol of 10% stretch at 1Hz, with no significant increase observed in the control group. Additionally, an increase in cardiac myofibril alignment, connexin 43 expression, and collagen type I distribution were noted. In this study we demonstrated the effectiveness of a new bioreactor design to improve contractility of engineered cardiac muscle tissue.