Identification of oxytocin expression in human and murine microglia
Identification of oxytocin expression in human and murine microglia
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人和小鼠小胶质细胞中催产素表达的鉴定
DOI：
10.1016/j.pnpbp.2022.110600
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发表时间：
2022
影响因子：
5.6
通讯作者：
Shimomura Kenju
中科院分区：
文献类型：
--
作者：
Maejima Yuko;Yokota Shoko;Ono Tomoyuki;Yu Zhiqian;Yamachi Megumi;Hidema Shizu;Nollet Kenneth E.;Nishimori Katsuhiko;Tomita Hiroaki;Yaginuma Hiroyuki;Shimomura Kenju
背景催产素是下丘脑合成的一种神经肽。除了在分娩和哺乳中的作用外，催产素还调节社会行为和伴侣关系。使用催产素来改变神经发育障碍（如自闭症谱系障碍）的行为的可能性具有临床意义。小胶质细胞是组织驻留的巨噬细胞，在神经发生、突触修剪和脑内稳态的免疫介导中起作用。最近，人们发现催产素可以减弱小胶质细胞促炎细胞因子的分泌，但这种催产素的来源尚未确定。这促使我们调查是否小胶质细胞本身的sources.MethodsWe研究催产素的表达在人类和小鼠脑组织中的性别，使用免疫组化。在来自野生型和催产素敲除小鼠的分离的小鼠小胶质细胞中检查催产素mRNA表达和分泌。此外，催产素和细胞因子的分泌测定在培养的小胶质细胞（MG 6）刺激与脂多糖（LPS）。ResultsWe确定催产素的表达在人脑组织，培养的小胶质细胞（MG 6），和原代小鼠小胶质细胞。此外，LPS刺激增加催产素mRNA的表达在原代小鼠小胶质细胞和MG 6细胞，催产素分泌以及。结论小胶质细胞分泌催产素与IL-1β、IL-10呈正相关。在功能上，催产素可能调节炎症细胞因子从小胶质细胞的旁分泌/自分泌的方式释放。
BackgroundOxytocin is a neuropeptide synthesized in the hypothalamus. In addition to its role in parturition and lactation, oxytocin mediates social behavior and pair bonding. The possibility of using oxytocin to modify behavior in neurodevelopmental disorders, such as autism spectrum disorder, is of clinical interest. Microglia are tissue-resident macrophages with roles in neurogenesis, synapse pruning, and immunological mediation of brain homeostasis. Recently, oxytocin was found to attenuate microglial secretion of proinflammatory cytokines, but the source of this oxytocin was not established. This prompted us to investigate whether microglia themselves were the source.MethodsWe examined oxytocin expression in human and murine brain tissue in both sexes using immunohistochemistry. Oxytocin mRNA expression and secretion were examined in isolated murine microglia from wild type and oxytocin-knockout mice. Also, secretion of oxytocin and cytokines was measured in cultured microglia (MG6) stimulated with lipopolysaccharide (LPS).ResultsWe identified oxytocin expression in microglia of human brain tissue, cultured microglia (MG6), and primary murine microglia. Furthermore, LPS stimulation increased oxytocin mRNA expression in primary murine microglia and MG6 cells, and oxytocin secretion as well. A positive correlation between oxytocin and IL-1β, IL-10 secretion emerged, respectively.ConclusionThis may be the first demonstration of oxytocin expression in microglia. Functionally, oxytocin might regulate inflammatory cytokine release from microglia in a paracrine/autocrine manner.
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影响因子：
6
作者：
Coraci, IS;Husemann, J;El Khoury, JB
通讯作者：
El Khoury, JB
影响因子：
23.9
作者：
Sierra, Amanda;Encinas, Juan M.;Deudero, Juan J. P.;Chancey, Jessica H.;Enikolopov, Grigori;Overstreet-Wadiche, Linda S.;Tsirka, Stella E.;Maletic-Savatic, Mirjana
通讯作者：
Maletic-Savatic, Mirjana
催产素调节大鼠海马神经元和神经胶质标记物的表达。
影响因子：
0.6
作者：
T. Havranek;Z. Lestanova;Boris Mravec;Vladimír Štrbák;J. Bakos;Z. Bacova
通讯作者：
Z. Bacova
DOI：
10.1073/pnas.93.21.11699
发表时间：
1996-10-15
影响因子：
11.1
作者：
Nishimori, K;Young, LJ;Matzuk, MM
通讯作者：
Matzuk, MM
瘦素通过 caspase 1 独立机制诱导大鼠小胶质细胞释放白介素-1β
影响因子：
4.7
作者：
E. Pinteaux;W. Inoue;Lorraine Schmidt;F. Molina;N. Rothwell;G. Luheshi
通讯作者：
G. Luheshi