Intravital Imaging of Thymopoiesis Reveals Dynamic Lympho-Epithelial Interactions
Intravital Imaging of Thymopoiesis Reveals Dynamic Lympho-Epithelial Interactions
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DOI：
10.1016/j.immuni.2011.12.016
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发表时间：
2012-02-24
期刊：
影响因子：
32.4
通讯作者：
Boehm, Thomas
中科院分区：
文献类型：
--
作者：
Hess, Isabel;Boehm, Thomas
T细胞发育发生在胸腺中。胸腺微环境吸引造血祖细胞，将它们指定为T细胞谱系，并协调它们的分化和进入外周。胸腺的解剖位置和小鼠胚胎的子宫内发育迄今为止排除了直接可视化的胸腺生成的初始步骤。在这里，我们描述了转基因斑马鱼线，使在体内观察胸腺生成。胸腺上皮细胞的细胞自主增殖，它们在与造血细胞接触时形态转化为网状网，以及胸腺沉降细胞的多种迁移途径可以直接可视化。出乎意料的动态胸腺归巢过程是趋化因子驱动的，不依赖于血液循环。胸腺细胞的发育似乎在不到4天内完成。我们的工作建立了一个通用的模型，在体内观察和操作的胸腺细胞。
T cell development occurs in the thymus. The thymic microenvironment attracts hematopoietic progenitors, specifies them toward the T cell lineage, and orchestrates their differentiation and egress into the periphery. The anatomical location of the thymus and the intrauterine development of mouse embryos have so far precluded a direct visualization of the initial steps of thymopoiesis. Here, we describe transgenic zebrafish lines enabling the in vivo observation of thymopoiesis. The cell-autonomous proliferation of thymic epithelial cells, their morphological transformation into a reticular meshwork upon contact with hematopoietic cells, and the multiple migration routes of thymus-settling cells could be directly visualized. The unexpectedly dynamic thymus homing process is chemokine driven and independent of blood circulation. Thymocyte development appears to be completed in less than 4 days. Our work establishes a versatile model for the in vivo observation and manipulation of thymopoiesis.