Transgenic cucumber plants harboring a rice chitinase gene exhibit enhanced resistance to gray mold (Botrytis cinerea)
Transgenic cucumber plants harboring a rice chitinase gene exhibit enhanced resistance to gray mold (Botrytis cinerea)
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DOI：
10.1007/s002990050371
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发表时间：
1998-01-01
影响因子：
6.2
通讯作者：
Akutsu, K
中科院分区：
文献类型：
--
作者：
Tabei, Y;Kitade, S;Akutsu, K
通过农杆菌介导，将CaMV 35S启动子驱动的水稻几丁质酶cDNA （RCC2）导入黄瓜。在添加了100 mg/l卡那霉素的MS培养基上再生了200多个转基因芽。60个细长的芽被检查为存在整合的RCC2基因，随后证实有它。其中，有20个菌株通过分生孢子感染灰霉菌（Botrytis cinerea）进行抗性测试：20个独立芽中有15个菌株的抗性高于对照（非转基因植株）。3个转基因黄瓜株系（CR29、CR32和CR33）对灰绿杆菌的抗性最高，病害在这些株系中完全被抑制。比较了高抗性转基因菌株CR32和CR33与易感菌株CR20和对照几丁质酶基因的表达情况。在高抗性品系中观察到不同的抗病反应。CR33抑制附着胞的形成和菌丝的渗透。虽然CR32允许菌丝穿透，但感染菌丝的侵袭受到限制。此外，CR32后代的分离比为3:1（抗性：易感）。由于对灰霉病的抗性具有遗传性，这些高抗性转基因黄瓜品系可作为优良的抗病育种材料。
A rice chitinase cDNA (RCC2) driven by the CaMV 35S promoter was introduced into cucumber (Cucumis sativus L.) through Agrobacterium mediation. More than 200 putative transgenic shoots were regenerated and grown on MS medium supplemented with 100 mg/l kanamycin. Sixty elongated shoots were examined for the presence of the integrated RCC2 gene and subsequently confirmed to have it. Of these, 20 were tested for resistance against gray mold (Botrytis cinerea) by infection with the conidia: 15 strains out of the 20 independent shoots exhibited a higher resistance than the control (non-transgenic plants). Three transgenic cucumber strains (designated CR29, CR32 and CR33) showed the highest resistance against B. cinerea: the spread of disease was inhibited completely in these strains. Chitinase gene expression in highly resistant transgenic strains (CR32 and CR33) was compared to that of a susceptible transgenic strain (CR20) and a control. Different responses for disease resistance were observed among the highly resistant strains. CR33 inhibited appressoria formation and penetration of hyphae. Although CR32 permitted penetration of hyphae, invasion of the infection hyphae was restricted. Furthermore, progenies of CR32 showed a segregation ratio of 3:1 (resistant:susceptible). As the disease resistance against gray mold was confirmed to be inheritable, these highly resistant transgenic cucumber strains would serve as good breeding materials for disease resistance.