Activation of therminal components of human complement by a trypsin-activated complex of human factor B and cobra venom factor.
Activation of therminal components of human complement by a trypsin-activated complex of human factor B and cobra venom factor.
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通过胰蛋白酶激活的人因子 B 和眼镜蛇毒因子复合物激活人补体的热成分。
DOI：
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发表时间：
1976
期刊：
影响因子：
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通讯作者：
S. Kashiba
中科院分区：
文献类型：
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作者：
A. Miyama;T. Kato;I. Minoda;T. Ueda;S. Kashiba
通过溶血、免疫电泳和免疫粘附反应证明了 CVF-B 对 C3 的裂解。免疫电泳未检测到 C5 裂解，但用 EAC1423 测定，C5 溶血活性降低，尽管低于 C3 溶血活性。 C3与有限量的C5的共存并没有降低晚效成分C6至C9和CVF-B诱导的豚鼠红细胞(GPE)的最终溶血程度。因此，由GPE、C5至C9和CVF-B组成的CVF-B溶血系统提供了滴定人补体末端成分的方法。 CVF-B能够通过激活C5、C6和C7在GPE上产生C567的溶血活性位点。液相中的复合物 C567 在 1 分钟内衰减，但 GPE 上的 C567 相当稳定。如果存在 C3b 位点，最初不敏感的绵羊红细胞会变得对 CVF-B 溶血系统敏感，这表明细胞结合的 C3b 在定向 C567 位置固定方面发挥了作用。可以从反应混合物的上清液中定量回收CVF-B，其中通过C5至C8与CVF-B之间的相互作用形成溶血活性中间体GPEC-5678。
Cleavage of C3 by CVF-B was demonstrated by hemolytic, immunoelectrophoretic and immune adherence reactions. No cleavage of C5 was detected by immunoelectrophoresis, but C5 hemolytic activity, assayed with EAC1423, decreased although less than C3 hemolytic activity. The co-existence of C3 with limiting amounts of C5 did not reduce the final degree of hemolysis of guinea pig erythrocytes (GPE) induced by late-acting components C6 through C9 and CVF-B. Thus, a CVF-B hemolytic system composed of GPE, C5 through C9 and CVF-B provided a method for titration of terminal components of human complement. CVF-B was able to generate hemolytically active sites of C567 on GPE by activation of C5, C6 and C7. The complex C567 in the fluid-phase decayed within 1 min but C567 on GPE was quite stable. Originally insensitive sheep erythrocytes became sensitive to the CVF-B hemolytic system if C3b sites were present, suggesting that cell-bound C3b played a role in orienting the positions of C567 to be fixed. CVF-B could be recovered quantitatively from the supernatant of the reaction mixture in which the hemolytically active intermediate GPEC-5678 had been formed through the interaction between C5 to C8 and CVF-B.