Biological inventory of Ranomafana National Park tetrapods using leech-derived iDNA
Biological inventory of Ranomafana National Park tetrapods using leech-derived iDNA
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DOI：
10.1007/s10344-019-1305-3
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发表时间：
2019-08-09
影响因子：
2
通讯作者：
Siddall, Mark
中科院分区：
文献类型：
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作者：
Fahmy, Mai;Ravelomanantsoa, Ny Anjara Fifi;Siddall, Mark
在印度-太平洋地区有大量的血蛭科陆生吸血水蛭，该地区包括世界上许多生物多样性热点。已证明，蛭形绦虫在其肠道中保留了数月数量级的高质量宿主DNA，并已成为热带雨林脊椎动物生物多样性评估的目标工具，那里的物种难以监测。补充以前的哺乳动物特有的16 S mtDNA数据，12 S mtDNA位点优化的四足动物被用来分配身份水蛭主机。通过传统的桑格测序的每一个16 S和12 S线粒体区域的每一个血粉，我们发现一个41%的增加，使用这两个基因座比单独使用16 S检测到的类群的多样性。除了通过16 S测序分配的哺乳动物多样性之外，通过两个位点的测序确定的宿主身份还告知了采样地点的多样性以及水蛭本身的觅食行为。我们提出的证据通才觅食之间的陆地水蛭马达加斯加（Chtonobdella fallax），数据表明哺乳动物，鸟类，两栖动物和爬行动物作为可行的水蛭主机。
The terrestrial blood feeding leeches of family Haemadipsidae are abundant throughout the Indo-Pacific, a region which encompasses many of the world's biodiversity hotspots. Haemadipsids have been shown to retain high-quality host DNA in their guts on the order of months and have been targeted as tools for vertebrate biodiversity assessment in tropical rainforests, where species are difficult to monitor. Complementing prior mammal-specific 16S mtDNA data, the 12S mtDNA locus optimized for tetrapods was employed to assign identities to leech hosts. Through traditional Sanger sequencing of each blood meal for each of 16S and 12S mitochondrial regions, we find a 41% increase in the diversity of taxa detected using both loci than of using 16S alone. In addition to mammalian diversity assigned through sequencing of 16S, the host identities determined through sequencing of both loci inform the diversity of sampled localities as well as the foraging behavior of the leeches themselves. We present evidence for generalist foraging among the terrestrial leeches of Madagascar (Chtonobdella fallax), with data suggesting mammals, birds, amphibians, and reptiles as viable leech hosts.