Papillomavirus capsid proteins mutually impact structure
Papillomavirus capsid proteins mutually impact structure
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DOI：
10.1016/j.virol.2011.01.018
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发表时间：
2011-04-10
期刊：
影响因子：
3.7
通讯作者：
Meyers, Craig
中科院分区：
文献类型：
--
作者：
Chen, Horng-Shen;Conway, Michael J.;Meyers, Craig
我们研究了一组含有野生型和嵌合衣壳HPV16和HPV18蛋白的突变病毒。突变体衣壳蛋白的表达，基因组扩增，和附加型的维护与野生型病毒。然而，嵌合病毒的滴度与野生型不同。我们表明，典型的突变嵌合衣壳病毒，L2影响的L1和L1的结构影响的L2的结构中的病毒粒子。使用一组构象依赖性中和L1单克隆抗体和L2衣壳表面肽衍生的中和抗体测量这些效应。这些数据表明，一个衣壳基因的变异不仅影响其自身的结构和抗原性，而且还影响另一个衣壳蛋白的结构和抗原性。我们的数据表明，为了使疫苗持续有效，需要考虑两种衣壳蛋白的变异，而不仅仅是疫苗所针对的蛋白。(C)2011 Elsevier Inc. All rights reserved.
We studied a panel of mutant viruses containing wild-type and chimeric capsid HPV16 and HPV18 proteins. The mutant capsid protein expression, genome amplification, and episomal maintenance were comparable with the wild-type virus. However, the chimeric viruses varied in their titers from wild-type. We show that the intertypical mutant chimeric capsid viruses, that L2 affects the structure of L1 and that L1 affects the structure of L2 in the virion. These effects were measured using a panel of conformation-dependent neutralizing L1 MAbs and an L2 capsid surface peptide derived neutralizing antibody. These data suggest that variation of one capsid gene not only affects its own structure and antigenicity, but also affects the structure and antigenicity of the other capsid protein. Implications of our data suggest that for the continued effectiveness of a vaccine, variation in both capsid proteins need to be considered and not just the protein the vaccine is directed against. (C) 2011 Elsevier Inc. All rights reserved.