Expression of influenza A virus internal antigens on the surface of infected P815 cells.
Expression of influenza A virus internal antigens on the surface of infected P815 cells.
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DOI：
10.4049/jimmunol.126.5.1814
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发表时间：
1981-05
影响因子：
4.4
通讯作者：
J. Yewdell;E. Frank;W. Gerhard
中科院分区：
文献类型：
--
作者：
J. Yewdell;E. Frank;W. Gerhard
一组杂交瘤抗体用于研究甲型流感病毒感染的P815细胞表面上的2种主要内部甲型流感病毒蛋白质、基质蛋白（M）和核蛋白（NP）的表达。使用灵敏的放射免疫测定法（RIA）进行活感染的P815细胞只有微量的M是可检测的，而M在其他3个细胞表面检测未检测到。相比之下，通过RIA检测到NP的量比M大10倍，比病毒糖蛋白低10- 50倍。此外，NP的存在可以通过对活感染细胞进行的免疫荧光、微量细胞毒性和凝集试验来证明。
A panel of hybridoma antibodies was used to study the expression of the 2 major internal influenza A virus proteins, matrix protein (M), and nucleoprotein (NP) on the surface of influenza A-infected P815 cells. Using a sensitive radioimmunoassay (RIA) performed with live infected P815 cells only minute amounts of M were detectable, whereas M was not detected in 3 other cell-surface assays. In contrast, NP was detected in 10-fold greater amounts than M and 10- to 50-fold lower amounts than the viral glycoproteins by RIA. Furthermore, the presence of NP could be demonstrated by immunofluorescence, microcytotoxicity, and agglutination assays performed on live infected cells.