The membrane-anchored BOTRYTIS-INDUCED KINASE1 plays distinct roles in Arabidopsis resistance to necrotrophic and biotrophic pathogens
The membrane-anchored BOTRYTIS-INDUCED KINASE1 plays distinct roles in Arabidopsis resistance to necrotrophic and biotrophic pathogens
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DOI：
10.1105/tpc.105.035576
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发表时间：
2006-01-01
期刊：
影响因子：
11.6
通讯作者：
Mengiste, T
中科院分区：
文献类型：
--
作者：
Veronese, P;Nakagami, H;Mengiste, T
植物对疾病的抗性是由防御反应途径的组合控制的，这些防御反应途径取决于病原体的性质而被激活。我们确定了拟南芥葡萄孢诱导激酶1（BIK1）基因，该基因受灰葡萄孢感染的转录调控。BIK1的失活导致对坏死营养型真菌病原体的严重易感性，但增强了对细菌病原体假单胞菌番茄致病变种的强毒株的抗性。对无毒细菌菌株的反应是不变的，BIK 1的作用限制在基础防御而不是种族特异性抗性。茉莉酸和乙烯调节的防御反应，通常与耐坏死性真菌，是衰减的bik1突变体的基础上的植物防御素PDF1.2基因的表达。bik1突变体显示改变的根生长，产生更多和更长的根毛，表明BIK1也是正常植物生长和发育所必需的。而bik1的病原体反应主要依赖于水杨酸（SA）水平，非防御反应是独立的SA。BIK1是膜定位的，表明可能参与病原体应答的识别或转导的早期阶段。我们的数据表明，BIK1调节防御反应所需的病原体感染和正常的根毛生长的细胞因子的信号转导，连接防御反应调节与生长和发育。
Plant resistance to disease is controlled by the combination of defense response pathways that are activated depending on the nature of the pathogen. We identified the Arabidopsis thaliana BOTRYTIS-INDUCED KINASE1 (BIK1) gene that is transcriptionally regulated by Botrytis cinerea infection. Inactivation of BIK1 causes severe susceptibility to necrotrophic fungal pathogens but enhances resistance to a virulent strain of the bacterial pathogen Pseudomonas syringae pv tomato. The response to an avirulent bacterial strain is unchanged, limiting the role of BIK1 to basal defense rather than race-specific resistance. The jasmonate- and ethylene-regulated defense response, generally associated with resistance to necrotrophic fungi, is attenuated in the bik1 mutant based on the expression of the plant defensin PDF1.2 gene. bik1 mutants show altered root growth, producing more and longer root hairs, demonstrating that BIK1 is also required for normal plant growth and development. Whereas the pathogen responses of bik1 are mostly dependent on salicylic acid ( SA) levels, the nondefense responses are independent of SA. BIK1 is membrane- localized, suggesting possible involvement in early stages of the recognition or transduction of pathogen response. Our data suggest that BIK1 modulates the signaling of cellular factors required for defense responses to pathogen infection and normal root hair growth, linking defense response regulation with that of growth and development.