Chitosan oligosaccharide induces resistance to Tobacco mosaic virus in Arabidopsis via the salicylic acid-mediated signalling pathway.
Chitosan oligosaccharide induces resistance to Tobacco mosaic virus in Arabidopsis via the salicylic acid-mediated signalling pathway.
复制标题
壳寡糖通过水杨酸介导的信号通路诱导拟南芥对烟草花叶病毒的抗性
DOI：
10.1038/srep26144
复制
发表时间：
2016-05-18
影响因子：
4.6
通讯作者：
Yin H
中科院分区：
文献类型：
--
作者：
Jia X;Meng Q;Zeng H;Wang W;Yin H
壳聚糖是世界上最丰富的碳水化合物生物聚合物之一，而由壳聚糖制备的壳寡糖（COS）是一种植物免疫调节剂。本研究旨在验证COS对拟南芥诱导抗烟草花叶病毒（TMV）的效果，并探究所涉及的潜在防御相关信号通路。在拟南芥中诱导TMV抗性的最佳条件是在接种TMV前1天用50mg/L的COS预处理。包括表型数据和TMV外壳蛋白表达在内的多层次指标显示，COS在野生型和茉莉酸途径缺陷型（jar1）拟南芥植株中诱导了对TMV的抗性，但在水杨酸途径缺陷型（NahG）拟南芥植株中没有。定量PCR和植物激素水平分析证实，COS预处理增强了防御相关基因PR1的表达，PR1是水杨酸信号通路的一个标志物，并且增加了野生型和jar1中水杨酸的量，但在NahG植株中没有。综上所述，这些结果证实COS通过激活水杨酸信号通路在拟南芥中诱导对TMV的抗性。
Chitosan is one of the most abundant carbohydrate biopolymers in the world, and chitosan oligosaccharide (COS), which is prepared from chitosan, is a plant immunity regulator. The present study aimed to validate the effect of COS on inducing resistance to tobacco mosaic virus (TMV) in Arabidopsis and to investigate the potential defence-related signalling pathways involved. Optimal conditions for the induction of TMV resistance in Arabidopsis were COS pretreatment at 50 mg/L for 1 day prior to inoculation with TMV. Multilevel indices, including phenotype data, and TMV coat protein expression, revealed that COS induced TMV resistance in wild-type and jasmonic acid pathway- deficient (jar1) Arabidopsis plants, but not in salicylic acid pathway deficient (NahG) Arabidopsis plants. Quantitative-PCR and analysis of phytohormone levels confirmed that COS pretreatment enhanced the expression of the defence-related gene PR1, which is a marker of salicylic acid signalling pathway, and increased the amount of salicylic acid in WT and jar1, but not in NahG plants. Taken together, these results confirm that COS induces TMV resistance in Arabidopsis via activation of the salicylic acid signalling pathway.