The Notch pathway regulates both the proliferation and differentiation of follicular cells in the panoistic ovary of Blattella germanica.
The Notch pathway regulates both the proliferation and differentiation of follicular cells in the panoistic ovary of Blattella germanica.
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DOI：
10.1098/rsob.150197
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发表时间：
2016-01
期刊：
影响因子：
5.8
通讯作者：
Piulachs MD
中科院分区：
文献类型：
--
作者：
Irles P;Elshaer N;Piulachs MD
Notch通路是发育过程中细胞增殖和分化的重要调节因子。它参与昆虫卵子发生已被检查在昆虫物种与meroistic卵巢，它是已知的细胞命运的决定和诱导的有丝分裂到内循环开关的滤泡细胞（FC）中发挥重要作用。本文报道了Notch途径的主要组分（Notch及其配体Delta和Serrate）在德国小蠊卵子发生中的功能。如基于RNAi的分析所揭示的，Notch和Delta被发现有助于将FC维持在不成熟的非凋亡状态。Notch的这种祖先功能似乎与Notch在果蝇中诱导从有丝分裂到内循环的转变相反，Notch功能的变化可能与昆虫卵巢类型的进化一致。Notch也被证明通过调节细胞骨架在诱导卵泡延长中发挥积极作用。此外，Delta和Notch相互作用被认为是确定FC后群体的分化。锯齿水平被认为是刻缺蛋白依赖性的，并参与FC程序的控制，虽然他们似乎在panoistic卵巢卵子发生中没有发挥关键作用。
The Notch pathway is an essential regulator of cell proliferation and differentiation during development. Its involvement in insect oogenesis has been examined in insect species with meroistic ovaries, and it is known to play a fundamental role in cell fate decisions and the induction of the mitosis-to-endocycle switch in follicular cells (FCs). This work reports the functions of the main components of the Notch pathway (Notch and its ligands Delta and Serrate) during oogenesis in Blattella germanica, a phylogenetically basal species with panoistic ovary. As is revealed by RNAi-based analyses, Notch and Delta were found to contribute towards maintaining the FCs in an immature, non-apoptotic state. This ancestral function of Notch appears in opposition to the induction of transition from mitosis to endocycle that Notch exerts in Drosophila melanogaster, a change in the Notch function that might be in agreement with the evolution of the insect ovary types. Notch was also shown to play an active role in inducing ovarian follicle elongation via the regulation of the cytoskeleton. In addition, Delta and Notch interactions were seen to determine the differentiation of the posterior population of FCs. Serrate levels were found to be Notch-dependent and are involved in the control of the FC programme, although they would appear to play no crucial role in panoistic ovary oogenesis.