Graphene nanogrids for selective and fast osteogenic differentiation of human mesenchymal stem cells
Graphene nanogrids for selective and fast osteogenic differentiation of human mesenchymal stem cells
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DOI：
10.1016/j.carbon.2013.03.010
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发表时间：
2013-08-01
期刊：
影响因子：
10.9
通讯作者：
Shahsavar, Mahla
中科院分区：
文献类型：
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作者：
Alzhavan, Omid;Ghaderi, Elham;Shahsavar, Mahla
石墨烯纳米网格（通过氧化解压缩多壁碳纳米管获得的石墨烯纳米带制备）被用作加速从脐带血中分离的人间充质干细胞（hMSCs）向成骨谱系分化的二维选择性模板。生物相容性和亲水的石墨烯纳米网格显示出与纳米网格模式一致的高肌动蛋白细胞骨架增殖。增殖量略高于亲水性氧化石墨烯（GO）片上的增殖量，显著高于疏水性还原氧化石墨烯（rGO）片和聚二甲基硅氧烷底物上的非均匀增殖量。在化学诱导剂的存在下，还原氧化石墨烯纳米带（rGONR）网格在7天的短时间内显示出高度加速的hMSCs成骨分化（模式分化），其成骨量比氧化石墨烯薄片上的分化（均匀分化）高2.2倍。我们发现，尽管在没有任何化学诱导剂的情况下，石墨烯纳米网格表现出轻微的成骨分化，但石墨烯片不能表现出任何分化。因此，rGONR网格上的高度加速分化归因于其对化学诱导剂的优异吸附能力和纳米网格表面形貌特征引起的物理应力。(C) 2013 Elsevier Ltd.版权所有。
Graphene nanogrids (fabricated by graphene nanoribbons obtained through oxidative unzipping of multi-walled carbon nanotubes) were used as two-dimensional selective templates for accelerated differentiation of human mesenchymal stem cells (hMSCs), isolated from umbilical cord blood, into osteogenic lineage. The biocompatible and hydrophilic graphene nanogrids showed high actin cytoskeleton proliferations coinciding with patterns of the nanogrids. The amounts of proliferations were found slightly better than proliferation on hydrophilic graphene oxide (GO) sheets, and significantly higher than non-uniform proliferations on hydrophobic reduced graphene oxide (rGO) sheets and polydimethylsiloxane substrate. In the presence of chemical inducers, the reduced graphene oxide nanoribbon (rGONR) grid showed a highly accelerated osteogenic differentiation of the hMSCs (a patterned differentiation) in short time of 7 days in which the amount of the osteogenesis was similar to 2.2 folds greater than the differentiation (a uniform differentiation) on the rGO sheets. We found that although in the absence of any chemical inducers the graphene nanogrids showed slight patterned osteogenic differentiations, the graphene sheets could not present any differentiation. Therefore, the highly accelerated differentiation on the rGONR grid was assigned to both its excellent capability in adsorption of the chemical inducers and physical stresses induced by the surface topographic features of the nanogrids. (C) 2013 Elsevier Ltd. All rights reserved.