Effects of a highly purified fucoidan from Undaria pinnatifida on growth performance and intestine health status of gibel carp Carassius auratus gibelio
Effects of a highly purified fucoidan from Undaria pinnatifida on growth performance and intestine health status of gibel carp Carassius auratus gibelio
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裙带菜高纯度岩藻依聚糖对银鲫生长性能和肠道健康的影响
DOI：
10.1111/anu.12966
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发表时间：
2020-02-01
影响因子：
3.5
通讯作者：
Zhang, Ming
中科院分区：
文献类型：
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作者：
Cui, Hao;Wang, Zhaoguang;Zhang, Ming
通过6周的饲养试验，研究了不同浓度裙带菜褐藻糖胶（1 g/kg、10 g/kg和30 g/kg; w/w）对异育银鲫（Carassius auratus gibelio）的影响。结果表明，30 g/kg褐藻糖胶显著提高了猪的生长性能、肠道消化酶活性、酸性磷酸酶活性和免疫球蛋白M含量（p <0.05）。组织学检查显示，与对照处理相比，接受30 g/kg岩藻聚糖硫酸酯的异育银鲫在中肠和远端肠中具有显著更高丰度的含粘蛋白的杯状细胞（p <0.05）。肠道微生物群分析显示，30 g/kg岩藻依聚糖补充显著增加了（p < .05）Cetobacterium和气单胞菌属的丰度，但降低了（p < .05）病原菌邻单胞菌属和粘蛋白降解细菌Mucinivorans的流行率。此外，RNA-seq和RT-qPCR分析表明，30 g/kg岩藻依聚糖硫酸酯引起参与免疫调节（如白细胞介素-8和环氧合酶）、信号转导（如磷脂酰肌醇-4，5-二磷酸3-激酶和蛋白激酶B）和营养利用（麦芽糖酶-葡糖淀粉酶和毒蕈碱乙酰胆碱受体3）的基因表达的显著变化（p <0.05）。总之，目前的研究表明，岩藻多糖添加剂可以提高肠道消化酶的活性，调节肠道微生物群落，增强免疫准备状态，从而改善异育银鲫的生长性能和肠道健康状况。
A six-week feeding trial was conducted to determine the effects of different concentrations of fucoidan (1 g/kg, 10 g/kg and 30 g/kg; w/w) from Undaria pinnatifida on gibel carp (Carassius auratus gibelio). Our results demonstrated that 30 g/kg fucoidan significantly increased (p < .05) growth performance, intestinal digestive enzyme activities, acid phosphatase activity and immunoglobulin M content. Histological examinations revealed that gibel carp receiving 30 g/kg fucoidan had significant higher abundance of mucin-containing goblet cells in middle and distal intestine as compared with control treatment (p < .05). Intestinal microbiota analysis showed that 30 g/kg fucoidan supplementation significantly increased (p < .05) the abundance of Cetobacterium and Aeromonas, but lowered (p < .05) the prevalence of pathogenic bacteria Plesiomonas and a mucin-degrading bacterium Mucinivorans. Furthermore, RNA-seq and RT-qPCR analysis indicated that 30 g/kg fucoidan caused significant changes (p < .05) in the expression of genes involved in immune regulation (such as interleukin-8 and cyclooxygenase), signal transduction (such as phosphatidylinositol-4,5-bisphosphate 3-kinase and protein kinase B) and nutrition utilization (maltase-glucoamylase and muscarinic acetylcholine receptor 3). Together, the current study shows that fucoidan supplementation could elevate the activity of intestinal digestive enzymes, modulate intestinal microbial communities and potentiate a higher state of immune readiness, which might consequently improve growth performance and intestine health status of gibel carp.