PEG-based hydrogels with collagen mimetic peptide-mediated and tunable physical cross-links.
PEG-based hydrogels with collagen mimetic peptide-mediated and tunable physical cross-links.
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DOI：
10.1021/bm100465q
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发表时间：
2010-09-13
影响因子：
6.2
通讯作者：
Yu, S. Michael
中科院分区：
文献类型：
--
作者：
Stahl, Patrick J.;Romano, Nicole H.;Wirtz, Denis;Yu, S. Michael
组织支架的力学性能对细胞的形态和分化有重要影响。与二维基质相比，三维水凝胶的局部生物化学和机械性能由于几何限制而难以控制。我们设计了合成的3D水凝胶，其特征在于四臂聚（乙二醇）（PEG）和胶原蛋白模拟肽（CMP）的复合物，其通过CMP的热可逆三螺旋组装介导的物理交联形成水凝胶。在这里，我们提出了各种PEG-CMP 3D水凝胶的制造和它们的局部机械性能由粒子跟踪微观流变学确定。结果表明，CMP介导的物理交联可以通过改变凝胶的温度或通过加入竞争三螺旋形成的游离CMP来破坏。这允许调节本体和局部刚度以及在PEG-CMP水凝胶内产生刚度梯度，这证明了其作为编码用于组织形成的物理化学信号的新型支架的潜力。
Mechanical properties of tissue scaffolds have major effects on the morphology and differentiation of cells. In contrast to two-dimensional substrates, local biochemical and mechanical properties of three-dimensional hydrogels are difficult to control due to the geometrical confinement. We designed synthetic 3D hydrogels featuring complexes of four-arm poly(ethylene glycol) (PEG) and collagen mimetic peptides (CMPs) that form hydrogels via physical crosslinks mediated by thermally reversible triple helical assembly of CMPs. Here we present the fabrication of various PEG-CMP 3D hydrogels and their local mechanical properties determined by particle tracking microrheology. Results show that CMP mediated physical crosslinks can be disrupted by altering the temperature of the gel or by adding free CMPs that compete for triple helix formation. This allowed modulation of both bulk and local stiffness as well as the creation of stiffness gradients within the PEG-CMP hydrogel, which demonstrates its potential as a novel scaffold for encoding physico-chemical signals for tissue formation.
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