Western blot (immunoblot) analysis of the fimbrial antigens of Bacteroides nodosus
Western blot (immunoblot) analysis of the fimbrial antigens of Bacteroides nodosus
复制标题
结节拟杆菌菌毛抗原的蛋白质印迹（免疫印迹）分析
DOI：
10.1128/jb.169.9.4018-4023.1987
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发表时间：
1987
影响因子：
3.2
通讯作者：
J. Egerton
中科院分区：
文献类型：
--
作者：
B. J. Anderson;J. Mattick;P. Cox;C. Kristo;J. Egerton
通过免疫印迹(Western blot)-抗体结合研究，从代表不同血清群和血清型的广泛菌株中分离出的菌毛，研究了菌毛亚基和假定的基础蛋白抗原在结节拟杆菌（Bacteroides nodsus）血清学分类中的作用。针对同源血清群的抗血清能识别毛状亚基，但通常不能被异源抗血清识别，而对基础抗原的识别与血清学分类无关。纤维亚基之间的二次交叉反应模式表明，一些血清群可能比其他血清群更密切相关。例子包括血清型C和G以及血清型D和h。对分类为血清型A1和A2的分离株的类似分析，以及血清型特异性抗血清，表明这种细分也是由毛状亚基决定的，即使在这一水平上也会发生显著变化。这些研究表明，结核分枝杆菌的不同血清组和血清型由一系列重叠的抗原相关菌株组成。
The roles of the fimbrial subunit and the putative basal protein antigens in the serological classification of Bacteroides nodosus have been examined by Western blot (immunoblot)-antibody binding studies of fimbriae isolated from a wide range of strains representative of different serogroups and serotypes. Fimbrial subunits were recognized by antiserum against the homologous serogroup but not generally by heterologous antisera, whereas recognition of the basal antigen was independent of serological classification. Secondary cross-reaction patterns among fimbrial subunits indicated that some serogroups may be more closely related than others. Examples include serogroups C and G and serogroups D and H. Similar analyses of isolates classified within serotypes A1 and A2, with serotype-specific antisera, showed that this subdivision is also determined by the fimbrial subunit and that significant variation does occur even at this level. These studies suggest that the various serogroups and serotypes of B. nodosus comprise a series of overlapping sets of antigenically related strains.