Two different snoRNAs are encoded in introns of amphibian and human L1 ribosomal protein genes.
Two different snoRNAs are encoded in introns of amphibian and human L1 ribosomal protein genes.
复制标题
两栖动物和人类 L1 核糖体蛋白基因的内含子编码两种不同的 snoRNA。
DOI：
10.1093/nar/21.25.5824
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发表时间：
1993
影响因子：
14.9
通讯作者：
I. Bozzoni
中科院分区：
文献类型：
--
作者：
S. Prislei;A. Michienzi;C. Presutti;P. Fragapane;I. Bozzoni
我们之前报道过，非洲猪瘟L1核糖体蛋白基因的第三个内含子编码一种名为U16的snoRNA。在这里，我们发现，同一基因的四个不同的内含子含有另一个以前未表征的snoRNA（U18），这是与原纤维蛋白在核仁中，并起源于前mRNA的加工。U18 RNA从前mRNA释放的途径与U16所述的途径相同：U18编码区上游和下游的初级核酸内切裂解产生前U18 RNA，随后将其修剪为成熟形式。U18的基因结构和加工在热带爪蟾和智人的相应基因中是保守的。因此，L1基因具有在进化中高度保守的复合结构，其中编码核糖体蛋白的序列与编码两种不同snoRNA的序列混合。这些不同组分的核仁定位表明它们在核糖体生物合成中具有某些共同的功能。
We previously reported that the third intron of the X.laevis L1 ribosomal protein gene encodes for a snoRNA called U16. Here we show that four different introns of the same gene contain another previously uncharacterized snoRNA (U18) which is associated with fibrillarin in the nucleolus and which originates by processing of the pre-mRNA. The pathway of U18 RNA release from the pre-mRNA is the same as the one described for U16: primary endonucleolytic cleavages upstream and downstream of the U18 coding region produce a pre-U18 RNA which is subsequently trimmed to the mature form. Both the gene organization and processing of U18 are conserved in the corresponding genes of X.tropicalis and H.sapiens. The L1 gene thus has a composite structure, highly conserved in evolution, in which sequences coding for a ribosomal protein are intermingled with sequences coding for two different snoRNAs. The nucleolar localization of these different components suggests some common function on ribosome biosynthesis.