Comparative proteomics of umbilical vein blood plasma from normal and gestational diabetes mellitus patients reveals differentially expressed proteins associated with childhood obesity
Comparative proteomics of umbilical vein blood plasma from normal and gestational diabetes mellitus patients reveals differentially expressed proteins associated with childhood obesity
复制标题
正常和妊娠糖尿病患者脐静脉血浆的比较蛋白质组学揭示了与儿童肥胖相关的差异表达蛋白质
DOI：
10.1002/prca.201600046
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发表时间：
2016-11-01
影响因子：
2
通讯作者：
Shi, Zhonghua
中科院分区：
文献类型：
--
作者：
Miao, Zhijing;Wang, Jianqing;Shi, Zhonghua
目的子代肥胖是妊娠期糖尿病(GDM)的长期并发症之一。本研究的目的是找出脐静脉血浆中差异表达的蛋白质，以作为早期诊断儿童肥胖的标志。实验设计收集了2007-2008年间30名对照组和30名GDM患者的脐静脉血浆样本，他们的子女在6-7岁时患有肥胖症。采用多重等压串联质量标记结合LC-MS/MS鉴定差异表达蛋白质。通过独创性途径分析来确定典型途径、生物学功能和相互作用的蛋白质网络。结果共鉴定出318个蛋白质，其中12个在GDM组上调，24个在GDM组下调。通过独创性途径分析，脂类代谢是最重要的类别。随机选择3种蛋白质进行Western blotting验证，结果与LC-MS结果一致。结论正常孕妇和肥胖孕妇脐静脉血浆蛋白质谱存在显著差异。结果表明，多种蛋白质和生物机制可能与儿童肥胖有关。
PurposeOffspring obesity is one of long-term complications of gestational diabetes mellitus (GDM). The aim of this study is to identify proteins differentially expressed in the umbilical vein blood plasma, which could become markers for early diagnosis of childhood obesity.Experimental designUmbilical vein plasma samples were collected from 30 control and 30 GDM patients in 2007-2008 whose offspring were suffering from obesity at 6-7 years old. Multiplexed isobaric tandem mass tag labeling combined with LC-MS/MS was used to identify differentially expressed proteins. Ingenuity pathway analysis was performed to identify canonical pathways, biological functions, and networks of interacting proteins. Western blotting was used to verify the expression of three selected proteins.ResultsA total of 318 proteins were identified, of which 12 proteins were upregulated in GDM group while 24 downregulated. Lipid metabolism was the top category identified by ingenuity pathway analysis. Three randomly chosen proteins were validated by Western blotting, which were consistent with LC-MS.ConclusionThere are significant differences of protein profile in the umbilical vein blood plasma between normal and GDM patients with obese offspring. The results indicate that a variety of proteins and biological mechanisms may contribute to childhood obesity.