Chondroitin sulfate N-acetylgalactosaminyltransferase-1 is required for normal cartilage development.
Chondroitin sulfate N-acetylgalactosaminyltransferase-1 is required for normal cartilage development.
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DOI：
10.1042/bj20100847
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发表时间：
2010-11-15
期刊：
影响因子：
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通讯作者：
Igarashi M
中科院分区：
文献类型：
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作者：
Watanabe Y;Takeuchi K;Higa Onaga S;Sato M;Tsujita M;Abe M;Natsume R;Li M;Furuichi T;Saeki M;Izumikawa T;Hasegawa A;Yokoyama M;Ikegawa S;Sakimura K;Amizuka N;Kitagawa H;Igarashi M
CS（硫酸软骨素）是广泛分布于细胞外基质中的糖胺聚糖种类。为了了解参与CS合成的酶的生理作用，我们产生了CSGalNAcT 1（CS N-乙酰氨基半乳糖转移酶1）-null小鼠。CS生产减少了大约一半CSGalNAcT 1-null小鼠，短链CS的量也减少了。此外，无效小鼠的软骨明显小于野生型小鼠。此外，在纯合突变小鼠中，发育中软骨中的II型胶原纤维异常聚集和紊乱。这些结果表明，CSGalNAcT 1是所需的正常CS生产在发展中的软骨。
CS (chondroitin sulfate) is a glycosaminoglycan species that is widely distributed in the extracellular matrix. To understand the physiological roles of enzymes involved in CS synthesis, we produced CSGalNAcT1 (CS N-acetylgalactosaminyltransferase 1)-null mice. CS production was reduced by approximately half in CSGalNAcT1-null mice, and the amount of short-chain CS was also reduced. Moreover, the cartilage of the null mice was significantly smaller than that of wild-type mice. Additionally, type-II collagen fibres in developing cartilage were abnormally aggregated and disarranged in the homozygous mutant mice. These results suggest that CSGalNAcT1 is required for normal CS production in developing cartilage.