The Disturbed Function of Neutrophils at the Early Stage of Fat Grafting Impairs Long-Term Fat Graft Retention
The Disturbed Function of Neutrophils at the Early Stage of Fat Grafting Impairs Long-Term Fat Graft Retention
复制标题
脂肪移植早期中性粒细胞功能紊乱会损害脂肪移植物的长期保留
DOI：
10.1097/prs.0000000000004882
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发表时间：
2018-11-01
影响因子：
3.6
通讯作者：
Lu, Feng
中科院分区：
文献类型：
--
作者：
Liu, Kaiyang;Cai, Junrong;Lu, Feng
背景：脂肪移植是一种常用的软组织填充方法；然而，其生存和再生的机制尚不完全清楚。中性粒细胞是前沿炎性细胞，与组织再生密切相关。为了了解中性粒细胞在脂肪移植保留中的作用，我们采用了中性粒细胞耗竭和上调模型。方法：小鼠腹股沟脂肪（约200mg）自体转移。在小鼠脂肪移植模型中分别使用抗小鼠Ly6G抗体和脂多糖进行中性粒细胞耗竭和活化。我们通过荧光激活细胞分选检测了操作组和对照组的血液和移植物间质血管部分。还比较了移植物重量、血管形成和分泌因子。结果：Ly6G抗体/脂多糖处理后，第7天前循环中性粒细胞计数和转移脂肪明显减少/增加。与对照组（51±10%,p < 0.05）相比，中性粒细胞的早期耗竭导致血管生成能力不足，最终保留率低（27±8%），而中性粒细胞的上调增加了炎症和活性氧水平，导致组织损伤和保留率低(20±9%，与对照组（51±10%,p < 0.05）相比。第4周后，中性粒细胞缺失组和上调组均可见巨噬细胞浸润增强。结论：未受干扰的中性粒细胞功能是启动巨噬细胞浸润下游反应、刺激血管形成和调节炎症水平的关键；因此，它对长期留存率有很大的影响。中性粒细胞功能紊乱，无论是增强还是减弱，都可能导致脂肪移植保留受损。
Background: Fat grafting is a popular soft-tissue filler method; however, the mechanism of its survival and regeneration is still not fully understood. Neutrophils are the frontier inflammatory cells and closely associated with tissue regeneration. To understand the role of neutrophils in fat graft retention, we adopted neutrophil depletion and up-regulation models. Methods: Mouse inguinal fat (approximately 200 mg) was transferred autologously. The anti-mouse Ly6G antibody and lipopolysaccharides were used in the mouse fat grafting model for neutrophil depletion or activation, respectively. We examined the blood and graft stromal vascular fraction by fluorescence-activated cell sorting in manipulation/control groups. Graft weight, vascularization, and secreted factors were also compared. Results: There was a significant reduction/increase of neutrophil counts in the circulation and the transferred fat before day 7 with Ly6G antibody/lipopolysaccharides treatment. Early depletion of neutrophils resulted in incompetent angiogenesis and eventually a poor retention rate (27 ± 8 percent) compared with control (51 ± 10 percent; p < 0.05), whereas up-regulated neutrophils increased the inflammation and reactive oxygen species level, leading to tissue damage and poor retention rate (20 ± 9 percent) compared with control (51 ± 10 percent; p < 0.05). Enhanced macrophage infiltration could be found in both neutrophil depletion and up-regulation groups after week 4. Conclusions: Undisturbed neutrophil function is the key to initiating downstream responses of macrophage infiltration, stimulating vessel formation, and regulating inflammation level; thus, it exerts a great impact on the long-term retention rate. Disturbed neutrophil function, either enhanced or weakened, can lead to impaired fat graft retention.