Direct isolation in cell culture of human rotaviruses and their characterization into four serotypes
Direct isolation in cell culture of human rotaviruses and their characterization into four serotypes
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人轮状病毒细胞培养中的直接分离及其四种血清型的表征
DOI：
10.1128/jcm.18.2.310-317.1983
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发表时间：
1983
影响因子：
9.4
通讯作者：
A. Kapikian
中科院分区：
文献类型：
--
作者：
R. Wyatt;H. James;A. Pittman;Y. Hoshino;H. Greenberg;A. Kalica;J. Flores;A. Kapikian
在检测的73份轮状病毒阳性粪便标本中，39份产生可在MA 104或原代非洲绿色猴肾细胞或两者中连续培养的人轮状病毒; 18份进行血清分型。通过空斑减少或试管中和试验或两者鉴定了四种不同的血清型，其中三种血清型与先前通过空斑减少建立的血清型相同，使用通过与可培养的牛轮状病毒的遗传重配培养的人轮状病毒。发现从日本收到的10株人轮状病毒株与我们代表这4种人轮状病毒血清型的候选原型株相似（如果不相同）。
Of 73 rotavirus-positive fecal specimens tested, 39 yielded a human rotavirus that could be cultivated serially in MA104 or primary African green monkey kidney cells or both; 18 were serotyped. Four distinct serotypes were identified by plaque reduction or tube neutralization assay or both, and three of these serotypes were the same as those established previously by plaque reduction, using human rotaviruses cultivated by genetic reassortment with a cultivable bovine rotavirus. Ten human rotavirus strains received from Japan were found to be similar, if not identical, to our candidate prototype strains representing these four human rotavirus serotypes.