Possible interaction of a Rho effector, Rhotekin, with a PDZ-protein, PIST, at synapses of hippocampal neurons
Possible interaction of a Rho effector, Rhotekin, with a PDZ-protein, PIST, at synapses of hippocampal neurons
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DOI：
10.1016/j.neures.2006.06.014
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发表时间：
2006-10-01
影响因子：
2.9
通讯作者：
Nagata, Koh-Ichi
中科院分区：
文献类型：
--
作者：
Ito, Hidenori;Iwamoto, Ikuko;Nagata, Koh-Ichi
Rhotekin是Rho的一种效应，在大脑中高度表达，但其功能几乎是未知的。在试图定义神经细胞中Rhotekin的性质，我们专注于它与极性相关分子的相互作用。在本研究中，我们提出了Rhotekin在体外与PDZ蛋白、与TC10特异性相互作用的PDZ结构域蛋白（PDZ domain protein，简称pte）相互作用的可能性，并发现这些蛋白在大鼠脑组织中形成复合物。然后，我们证明Rhotekin和ist在大鼠大脑中以特定发育阶段的方式表达。免疫荧光分析表明，Rhotekin和ist在大鼠原代培养的海马神经元突触上共定位。另一方面，发现在HEK293细胞和大鼠脑中，与另一种pdz结合蛋白β -catenin形成免疫复合物，并与该蛋白共定位于树突丝状足。Rhotekin的存在抑制了PIST与β -连环蛋白的相互作用。这些结果表明，Rhotekin与ist和β -连环蛋白在神经元细胞中的作用可能尚未确定。(c) 2006年爱思唯尔爱尔兰有限公司和日本神经科学学会。版权所有。
Rhotekin, an effector of Rho, is highly expressed in the brain but its function is almost unknown. In an attempt to define the properties of Rhotekin in neuronal cells, we focused on its interaction with polarity-related molecules. In the present study, we raised the possibility that Rhotekin interacts with a PDZ-protein, PIST (PDZ domain protein interacting specifically with TC10) in vitro, and found that these proteins form complex in the rat brain tissues. We then demonstrated that Rhotekin and PIST are expressed in developmental stage-specific manners in the rat brain. In immunofluorescence analyses, Rhotekin and PIST were suggested to co-localize at synapses in rat primary cultured hippocampal neurons. On the other hand, PIST was found to form immunocomplex with another PDZ-binding protein, beta-catenin, in HEK293 cells and in the rat brain, and co-localized with this protein at dendritic filopods. The interaction of PIST with beta-catenin was inhibited by the presence of Rhotekin. These results suggest a possible yet unidentified role of Rhotekin, in harmony with PIST and beta-catenin, in neuronal cells. (c) 2006 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.