Immunochemical localization of Clara cell protein by light and electron microscopy in conducting airways of fetal and neonatal hamster lung.
Immunochemical localization of Clara cell protein by light and electron microscopy in conducting airways of fetal and neonatal hamster lung.
复制标题
通过光学和电子显微镜对胎儿和新生儿仓鼠肺的气道进行免疫化学定位。
DOI：
10.1002/ar.1092270109
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
McDowell,EM
中科院分区：
文献类型：
--
作者：
Strum,JM;Singh,G;Katyal,SL;McDowell,EM
针对成年叙利亚金黄地鼠Clara细胞产生的一种蛋白质的抗体已被用来监测该物种传导呼吸道上皮中分泌细胞的发育和功能分化。研究了妊娠第11天的胎儿和新生仓鼠的肺，间隔至3.5周龄(包括成年仓鼠)。免疫过氧化物酶标记这种Clara细胞蛋白的最早时间是在妊娠第15天。在这一天，在气管内的少数分泌细胞、叶支气管内的许多分泌细胞以及几乎所有细支气管内的分泌细胞中都观察到了标记。纤毛细胞和内分泌细胞未被标记。在妊娠第15天，所有呼吸道水平的分泌细胞顶端胞浆中都出现了颗粒。为了确定该蛋白的确切亚细胞位置，采用了使用蛋白A金的超微结构标记程序。金颗粒仅标记分泌细胞内的电子致密颗粒，表明它们代表该蛋白质的特定位置。由于最远端传导呼吸道中的分泌细胞与较大呼吸道(包括气管)中的细胞在发育的同一天开始产生这种蛋白质，这种功能成熟的表达同时发生在整个传导呼吸道树中，而不是从头端到尾端顺序进行。因此，排列在较小传导通道内的分泌细胞比排列在较大通道上的分泌细胞成熟得更快。
An antibody to a protein produced by Clara cells in adult Syrian golden hamsters has been used to monitor the development and functional differentiation of secretory cells in the conducting airway epithelium of this species. Lungs from fetal and neonatal hamsters at gestational day 11 and at intervals up to and including 3.5 weeks of age (as well as adults) were studied. The earliest time this Clara cell protein could be identified by immunoperoxidase labeling in the fetal conducting airways was at gestational day 15. On this day, labeling was observed in a few secretory cells lining the trachea, in many lining the lobar bronchi, and in virtually all secretory cells lining the bronchioles. Ciliated cells and endocrine cells were not labeled. Granules first appeared within the apical cytoplasm of the secretory cells on gestational day 15 at all airway levels. To identify the exact subcellular location of this protein, an ultrastructural labeling procedure using protein A gold was employed. The gold particles labeled only electron‐dense granules within the secretory cells, indicating that they represent the specific site of this protein. Since secretory cells in the most distal conducting airways began to produce this protein on the same day in development as cells in the larger airways, including the trachea, this expression of functional maturation occurs simultaneously throughout the conducting respiratory tree rather than proceeding sequentially in a cranial to caudal direction. Consequently, secretory cells lining the smaller conducting airways mature more rapidly than those lining the larger airways.