Dietary n‐3 highly unsaturated fatty acids affect the biological and serum biochemical parameters, tissue fatty acid profile, antioxidation status and expression of lipid‐metabolism‐related genes in grass carp, Ctenopharyngodon idellus
Dietary n‐3 highly unsaturated fatty acids affect the biological and serum biochemical parameters, tissue fatty acid profile, antioxidation status and expression of lipid‐metabolism‐related genes in grass carp, Ctenopharyngodon idellus
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DOI：
10.1111/anu.12169
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发表时间：
2015-06
影响因子：
3.5
通讯作者：
Chaochao Li;Pin Liu;H. Ji;Ji-qin Huang;W. Zhang
中科院分区：
文献类型：
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作者：
Chaochao Li;Pin Liu;H. Ji;Ji-qin Huang;W. Zhang
通过95天的饲养试验，研究了n-3高不饱和脂肪酸(n-3 HUFA)对草鱼生长、抗氧化和脂肪代谢的影响。以猪油(LO)和鱼油(FO)为主要脂源，配制了两种等氮等脂饲料。结果表明，FO组大鼠腹膜脂肪(IPF)比例显著低于对照组(P<0.05)。FO组肌肉、肝胰腺和IPF中n-3HUFA浓度显著高于对照组(P<；0.05)。FO组血清低密度脂蛋白(LDL)含量显著降低(P<0.05)，血糖(GLU)含量显著升高(P<；0.05)。FO组大鼠血清总超氧化物歧化酶(T-SOD)活性显著升高(P<0.05)，与血清丙二醛(MDA)含量一致。与LO组比较，FO组IPF脂肪酸合成酶(Fas)、乙酰辅酶A羧基酶(Acc)、固醇调节元件结合蛋白-1(SREBP-1)和过氧化物酶体增殖物激活受体γ(PPARγ)基因表达显著降低(P<0.05)，而PPARα(PPARα)基因表达显著升高(P<0.05)。在肝胰腺中也发现了类似的趋势，但PPARγ除外。提示n-3HUFA可能通过影响脂代谢相关基因的表达来抑制草鱼体内脂类的积累。
A 95-day feeding trial was conducted to determine the effects of n-3 highly unsaturated fatty acids (n-3 HUFA) on the growth, antioxidation and lipid metabolism in grass carp (Ctenopharyngodon idellus). Two isonitrogenous and isolipidic diets were formulated with either lard oil (LO) or fish oil (FO) as the main lipid source. The results showed that the intraperitoneal fat (IPF) ratio was significantly lower (P < 0.05) in FO group. The concentration of n-3 HUFA in muscle, hepatopancreas and IPF was significantly higher in FO group (P < 0.05). The serum low-density lipoprotein (LDL) content was significantly lower (P < 0.05), and glucose (GLU) content was significantly higher (P < 0.05) in FO group. The serum total superoxide dismutase (T-SOD) activity was significantly higher (P < 0.05) in FO group, consistent with the serum malondialdehyde (MDA) content. The gene expression of IPF fatty acid synthase (FAS), acetyl-CoA carboxylase (ACC), sterol regulatory element-binding protein (SREBP-1) and peroxisome proliferator-activated receptor γ (PPARγ) was significantly lower (P < 0.05) and that of peroxisome proliferator-activated receptor α (PPARα) was significantly higher (P < 0.05) in FO group compared with LO group. Similar trends were found in the hepatopancreas, except for PPARγ. It is suggested that n-3 HUFA could inhibit lipid accumulation in grass carp by affecting the expression of lipid-metabolism-related genes.