Integration sites of human papillomavirus 18 DNA sequences on HeLa cell chromosomes.
Integration sites of human papillomavirus 18 DNA sequences on HeLa cell chromosomes.
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DOI：
10.1159/000132342
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发表时间：
1987
期刊：
影响因子：
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通讯作者：
N. Popescu;J. DiPaolo;S. Amsbaugh
中科院分区：
文献类型：
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作者：
N. Popescu;J. DiPaolo;S. Amsbaugh
人乳头瘤病毒（HPV）16和18与人类生殖器癌症密切相关。在大多数宫颈癌中，病毒序列整合到宿主基因组中。宫颈癌细胞系HeLa具有多个整合的HPV 18 DNA拷贝。在这项研究中，原位染色体杂交技术被用来分配的HPV 18 DNA序列在HeLa细胞染色体上的整合位点。在异常的5号染色体上的8 q23-q24、9 q31-q34、p11-p13和异常的22号染色体上的q12-q13处发现了4个杂交位点。这些位点中的三个与MYC、ABL和SIS原癌基因的位置相对应，并且位于或靠近脆性位点。HPV 18 DNA的染色体定位可能有助于评估病毒整合在这种恶性肿瘤发展中的作用。
Human papillomaviruses (HPV) 16 and 18 are closely linked with human genital cancer. In most cervical carcinomas, viral sequences are integrated into the host genome. HeLa, a cervical carcinoma cell line, has multiple copies of integrated HPV 18 DNA. In this study, in situ chromosome hybridization was used to assign the integration sites of HPV 18 DNA sequences on HeLa cell chromosomes. Four sites of hybridization were identified at 8q23----q24, 9q31----q34, p11----p13 on an abnormal chromosome 5, and q12----q13 on an abnormal 22. Three of these sites correspond with the locations of MYC, ABL, and SIS protooncogenes, and are at or in close proximity to fragile sites. The chromosomal localization of HPV 18 DNA may be useful in assessing the role of viral integration in the development of this malignancy.