Remarkable preservation of biomolecules in ancient radish seeds
Remarkable preservation of biomolecules in ancient radish seeds
复制标题
古代萝卜种子中生物分子的显着保存
DOI：
--
复制
发表时间：
1996
期刊：
影响因子：
--
通讯作者：
T. Brown
中科院分区：
文献类型：
--
作者：
K. O'Donoghue;A. Clapham;R. Evershed;T. Brown
对从埃及Qasr Ibrim回收的公元6世纪储存容器中干燥的种子进行检查，以确定脂质和核酸的存在。一个显着程度的脂质保存被发现，脂肪酸和甾醇的配置文件是非常相似的现代萝卜种子。唯一的显著差异是三酰甘油的水解和多不饱和脂肪酸（C18：2和C18：3）的消耗。主要脂肪酸的δ 13 C值在-25.4至-29.2%0/00范围内，考虑到最近大气CO2变化引起的同位素变化，这与现代萝卜（C3种子）一致。脱氧核糖核苷和核酸碱基检测直接化学分析，聚合酶链反应得到的产品与现代萝卜的序列相媲美。脂质保存的程度，这是远远大于其他考古遗迹的报告，表明干燥种子内的微环境延缓生物分子衰变。结果说明了实用程序相结合的脂质核酸分析化学分类学和基因型研究的考古植物遗骸。
Desiccated seeds from a 6th century AD storage vessel recovered from Qasr Ibrim, Egypt, were examined for the presence of lipids and nucleic acids. A remarkable degree of lipid preservation was discovered, the fatty acid and sterol profiles being very similar to those of modern radish seeds. The only significant differences were hydrolysis of triacylglycerols and depletion of the polyunsaturated fatty acids (C18:2 and C18:3). The δ13C values of the principal fatty acids were in the range –25.4 to –29.2%0/00, which is congruent with modern radish (C3 seeds) taking account of isotopic shifts caused by recent changes in atmospheric CO2. Deoxyribonucleosides and nucleic acid bases were detected by direct chemical analysis, and polymerase chain reactions gave products with sequences comparable to those from modern radish. The degree of lipid preservation, which was much greater than that reported for other archaeological remains, suggests that the microenvironment within desiccated seeds retards biomolecular decay. The results illustrate the utility of combined lipid-nucleic acid analysis in chemotaxonomic and genotypic studies of archaeobotanical remains.