Comparative scoring of micronucleated reticulocytes in rat peripheral blood by flow cytometry and microscopy.
Comparative scoring of micronucleated reticulocytes in rat peripheral blood by flow cytometry and microscopy.
复制标题
通过流式细胞术和显微镜对大鼠外周血中微核网织红细胞的比较评分。
DOI：
10.1093/toxsci/kfg143
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Dertinger,StephenD
中科院分区：
文献类型：
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作者：
Torous,DorotheaK;Hall,NikkiE;Murante,FrancisG;Gleason,SarahE;Tometsko,CarolR;Dertinger,StephenD
用流式细胞仪技术测量大鼠外周血中微核网织红细胞的发生率，并与基于显微镜的标准方法进行比较。在这些研究中，5组雄性SD大鼠接受了赋形剂或多种化学遗传毒物的处理：6-硫代鸟嘌呤、N-甲基-N‘-硝基-N-亚硝基、长春新碱、甲基氮杂环丙啶、乙醛、甲磺酸甲酯、苯、野百合碱和硫唑嘌呤。动物每天给药一次，持续2天，末次给药后24-48小时采集外周血。对这些样本进行流式细胞仪评分和基于显微镜的分析，采用活细胞上的吖啶橙染色，并测定每个样本的网织红细胞和微核网织红细胞的百分比。结果数据表明，这些评分方法之间有很好的一致性，尽管仔细执行流式细胞术方法可以通过减少评分时间和评分误差来增强微核检测。这些数据进一步支持了这样一个前提，即可以有效地利用大鼠的外周血室来检测遗传毒物诱导的微核。
A flow cytometric technique for scoring the incidence of micronucleated reticulocytes in rat peripheral blood was compared to a standard microscopy-based procedure. For these studies, groups of five male Sprague-Dawley rats were treated with vehicle or a broad range of chemical genotoxicants: 6-thioguanine,N-methyl-N‘-nitro-N-nitrosoguanidine, vincristine, methylaziridine, acetaldehyde, methyl methanesulfonate, benzene, monocrotaline, and azathioprine. Animals were treated once a day for up to 2 days, and peripheral blood was collected between 24 and 48 h after the final administration. These samples were processed for flow cytometric scoring and microscopy-based analysis using supravital acridine orange staining, and the percentage of reticulocytes and micronucleated reticulocytes was determined for each sample. The resulting data demonstrate good agreement between these scoring methodologies, although careful execution of the flow cytometric method was found to enhance the micronucleus assay by reducing both scoring time and scoring error. These data add further support to the premise that the peripheral blood compartment of rats can be used effectively to detect genotoxicant-induced micronuclei.