Effects of Nerve-Muscle Pedicle on Immobile Rat Vocal Folds in the Presence of Partial Innervation
Effects of Nerve-Muscle Pedicle on Immobile Rat Vocal Folds in the Presence of Partial Innervation
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DOI：
10.1177/000348941011901206
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发表时间：
2010-12
期刊：
影响因子：
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通讯作者：
T. Aoyama;Y. Kumai;E. Yumoto;Takaaki Ito;Satoru Miyamaru
中科院分区：
文献类型：
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作者：
T. Aoyama;Y. Kumai;E. Yumoto;Takaaki Ito;Satoru Miyamaru
目的：研究在部分喉返神经（RLN）神经支配的情况下，颈袢神经（ACN）肌蒂植入甲状腱肌（TA）肌是否有效。方法：对36只大鼠进行研究。12只大鼠作为阳性和阴性对照动物。在其余24只大鼠中，横断左侧RLN，切除1毫米神经，将残肢与硅胶管（STs）对接，诱导部分RLN再生。12只接受ST治疗的大鼠单独接受该手术，另外12只大鼠在ST治疗5周后将神经肌肉蒂（NMP）植入左TA肌。在15周时，通过TA肌的组织学评估和刺激RLNs和acn的肌电图来评估神经再生。结果：NMP组肌肉面积、神经末梢数量、乙酰胆碱受体数量、神经末梢与乙酰胆碱受体之比均显著高于ST组（p < 0.05）。肌电图在刺激rnn和acn时引起TA肌复合动作电位。结论：大鼠在部分RLN神经支配下，NMP植入可有效减轻TA肌的萎缩变化。
Objectives: We investigated whether implantation of an ansa cervicalis nerve (ACN)–muscle pedicle into the thyroarytenoid (TA) muscle is efficacious in the presence of partial recurrent laryngeal nerve (RLN) innervation. Methods: We studied a total of 36 rats. Twelve of the rats served as positive and negative control animals. In the remaining 24 rats, the left RLN was transected, a 1-mm piece of nerve was removed, and the stumps were abutted in silicone tubes (STs), inducing partial RLN regeneration. Twelve of the ST-treated rats underwent this procedure alone, and the other 12 rats had a nerve-muscle pedicle (NMP) implanted into the left TA muscle 5 weeks after ST treatment. At 15 weeks, reinnervation was assessed by histologic evaluation of the TA muscle and by electromyography with stimulation of the RLNs and ACNs. Results: The muscle area, the number of nerve terminals, the number of acetylcholine receptors, and the ratio of nerve terminals to acetylcholine receptors were significantly greater (p < 0.05) in the NMP group than in the ST group. Electromyography elicited TA muscle compound action potentials upon stimulation of the RLNs and ACNs. Conclusions: In rats, NMP implantation is efficacious for reducing atrophic changes in the TA muscle in the presence of partial RLN innervation.