Increased expression of Th2-associated chemokines in bullous pemphigoid disease.: Role of eosinophils in the production and release of these chemokines
Increased expression of Th2-associated chemokines in bullous pemphigoid disease.: Role of eosinophils in the production and release of these chemokines
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DOI：
10.1016/j.clim.2006.03.014
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发表时间：
2006-08-01
影响因子：
8.6
通讯作者：
Lamkhioued, Bouchaib
中科院分区：
文献类型：
--
作者：
Abdelilah, Soussi Gounni;Wellemans, Vincent;Lamkhioued, Bouchaib
大疱性类天疱疮是一种皮肤炎症性疾病，与嗜酸性粒细胞浸润和相关水泡液中存在高水平 Th2 细胞因子有关。关于趋化因子在这种疾病中的作用知之甚少。我们发现嗜酸性粒细胞趋化因子和 MCP-4 mRNA 和免疫反应性在 BP 患者的所有活检组织中均有表达，并且主要位于表皮和嗜酸性粒细胞。 IL-5处理后嗜酸性粒细胞中eotaxin和MCP-4的表达增强。随后用 Ig 免疫复合物刺激 IL-5 引发的嗜酸性粒细胞，导致上清液中嗜酸细胞趋化因子和 MCP-4 的分泌增加。使用免疫染色，这两种趋化因子定位于嗜酸性粒细胞颗粒。 BF被发现对嗜酸性粒细胞、中性粒细胞和T细胞具有趋化活性。当用IL-5或IL-4刺激嗜酸性粒细胞时，BF对嗜酸性粒细胞的趋化作用更有效。我们还发现 BF 中 Th-2 相关趋化因子（eotaxin 和 MCP-4）的水平显着高于 Th-1 相关趋化因子（MIP-1 beta 和 IP-10）。与 Th-1 分化的 T 细胞相比，这与极化 Th2 细胞对 BF 的趋化性增强一致。我们的结果支持 Th2 相关趋化因子参与 BP 疾病的发病机制。 (c) 2006 Elsevier Inc. 保留所有权利。
Bullous pemphigoid is an inflammatory disease of the skin associated with eosinophil infiltration and the presence of high levels of Th2 cytokines in the associated blister fluid. Little is known about the contribution of chemokines in this disease. We found that eotaxin and MCP-4 mRNA and immunoreactivity were expressed in all biopsies of BP patients and were mainly localized to the epidermis and eosinophils. The expression of eotaxin and MCP-4 was enhanced in eosinophils following IL-5 treatment. Subsequent stimulation of IL-5-primed eosinophils with Ig-immune complexes, results in increase secretion of eotaxin and MCP-4 in the supernatants. Using immunostaining, these two chemokines were localized to the granules of eosinophils. BF was found to contain chemotactic activity for eosinophils, neutrophils and T cells. The chemotactic effect of BF for eosinophils was more effective when eosinophils were stimulated with IL-5 or IL-4. We also found that the levels of Th-2-associated chemokines (eotaxin and MCP-4) in BF were significantly higher than the Th-1-associated chemokines (MIP-1 beta and IP-10). This was consistent with the increased chemotaxis of polarized Th2 cells toward BF, when compared to Th-1-differentiated Tcells. Our results support the involvement of Th2-associated chemokines in the pathogenesis of BP disease. (c) 2006 Elsevier Inc. All rights reserved.