Reticuloendothelial Clearance of Blood‐borne Particulates: Relevance to Experimental Lung Microembolization and Vascular Injury
Reticuloendothelial Clearance of Blood‐borne Particulates: Relevance to Experimental Lung Microembolization and Vascular Injury
复制标题
血源性颗粒的网状内皮清除率：与实验性肺微栓塞和血管损伤的相关性
作者：
G. Niehaus;P. Schumacker;T. Saba
在铜绿假单胞菌菌血症期间观察到的绵羊肺血管通透性的快速增加可能是由于血液传播颗粒栓塞肺微血管。由于绵羊轻度败血症期间肺微血管通透性的改变可能反映细菌的RES吞噬清除效率低下以及细菌诱导的血管内凝血产物，因此将绵羊（30-50 kg）RES功能的调理和吞噬方面与其他物种进行了比较。通过凝胶化I131 RE试验脂肪乳剂和凝胶化胶体碳的清除率和相对器官摄取率评价RES功能。免疫反应性调理素2SB糖蛋白水平测定电免疫法。绵羊中凝胶化（500 mg/kg）供试脂质RES清除的吞噬指数为0.019 ± 0.002，对应的半衰期为16.65 ± 1.74分钟。在胶体碳（64 mg/kg）的情况下，绵羊的吞噬指数为0.080 ± 0.026，对应于6.16 ± 1.99分钟的半衰期。主要RE器官中注射的脂肪乳剂百分比（%ID）（基于总器官（TO））为：肝脏= 15.69 ± 1.65%;脾脏= 2.09 ± 0.78%。肺内定位= 31.39 ± 6.2%。主要RE器官中碳的百分比（%ID/TO）为：肝脏= 21.37 ± 1.9%;脾脏= 1.95 ± 0.55%。肺内定位= 32.70 ± 4.55%。相比之下，在相同的相对挑战剂量下，大鼠和犬中血源性试验微粒的清除率和器官分布显示肝脏和脾脏RES摄取更强烈和快速，肺定位最小（1-2%）。免疫反应性调理蛋白的浓度变化很大，物种和RES功能的效率直接相关。观察到的水平为：犬= 1285
The rapid increase in sheep lung vascular permeability observed during Pseudomonas aeruginosa bacteremia may be due to embolization of the pulmonary microvasculature by blood-borne particulates. Since alterations in lung microvascular permeability during mild septicemia in sheep may reflect inefficient RES phagocytic clearance of bacteria as well as products of bacterial induced intravascular coagulation, the opsonic and phagocytic aspects of RES function in sheep (30–50 kg) were compared to other species. RES function was evaluated by both the clearance and relative organ uptake of gelatinized I131 RE test lipid emulsion and gelatinized colloidal carbon. Immunoreactive opsonic 2SB glycoprotein levels were determined by electroimmunoassay. The phagocytic index for RES clearance of the gelatinized (500 mg/kg) test lipid in sheep was 0.019 ± 0.002 corresponding to a half-time of 16.65 ± 1.74 minutes. With colloidal carbon (64 mg/kg), the phagocytic index in sheep was 0.080 ± 0.026, corresponding to a half-time of 6.16 ± 1.99 minutes. The per cent of injected lipid emulsion (%ID) in major RE organs, on a total organ basis (TO), was: liver = 15.69 ± 1.65%; spleen = 2.09 ± 0.78%. Localization in the lung = 31.39 ± 6.2%. The per cent of carbon localized in major RE organs (%ID/TO) was: liver = 21.37 ± 1.9%; spleen = 1.95 ± 0.55%. Localization in the lung = 32.70 ± 4.55%. In contrast, clearance and organ distribution of the blood-borne test microparticles in rats and dogs at the same relative challenging dose revealed a much more intense and rapid liver and spleen RES uptake with minimal lung localization (1–2%). Immunoreactive opsonic protein concentrations varied greatly with species and directly correlated with efficiency of RES function. Levels observed were: dog = 1285