ENCODED COMBINATORIAL CHEMISTRY
ENCODED COMBINATORIAL CHEMISTRY
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DOI：
10.1073/pnas.89.12.5381
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发表时间：
1992-06-15
影响因子：
11.1
通讯作者：
LERNER, RA
中科院分区：
文献类型：
--
作者：
BRENNER, S;LERNER, RA
化学合成的多样性和遗传学的力量相联系，为药物筛选提供了一个强大的，通用的方法。交替并行组合合成的过程用于编码具有独特核苷酸序列的大型化学品文库的单个成员。在化学实体与靶标结合后，可以通过复制扩增遗传标签，并通过与文库的子集的连续杂交来用于富集结合的分子。通过对核苷酸标签进行测序来解码与受体结合的化学结构的性质。
The diversity of chemical synthesis and the power of genetics are linked to provide a powerful, versatile method for drug screening. A process of alternating parallel combinatorial synthesis is used to encode individual members of a large library of chemicals with unique nucleotide sequences. After the chemical entity is bound to a target, the genetic tag can be amplified by replication and utilized for enrichment of the bound molecules by serial hybridization to a subset of the library. The nature of the chemical structure bound to the receptor is decoded by sequencing the nucleotide tag.