Induction of calprotectin mRNAs by lipopolysaccharide in the salivary gland of mice
Induction of calprotectin mRNAs by lipopolysaccharide in the salivary gland of mice
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DOI：
10.2152/jmi.56.287
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发表时间：
2009-12-01
影响因子：
0.7
通讯作者：
Hosoi, Kazuo
中科院分区：
文献类型：
--
作者：
Javkhlan, Purevjav;Hiroshima, Yuka;Hosoi, Kazuo
钙卫蛋白（Calprotectin）属于S100蛋白家族，是白细胞内主要的钙结合蛋白。S100 A8和S100 A9是钙卫蛋白的主要类型，是由轻链和重链亚基组成的异二聚体复合物。当宿主动物患有多种炎性疾病时，血浆、粪便、滑液、龈沟液、牙结石和唾液中的钙卫蛋白水平发生变化。Toll样受体（TLR）家族成员是脂多糖（LPS）等病原体的模式识别受体。在这里，我们研究了TLR受体的生物学作用是否反映在唾液腺中的钙卫蛋白表达。通过使用实时RT-PCR的时间过程研究检测到较高水平的S100 A8和S100 A9 mRNA在LPS注射后1.5-3 h在C3 H/HeN小鼠的下颌下腺（SMG）和腮腺（PG）中，但在TLR-4突变株C3 H/HeJ的相同组织中，表明这种诱导是通过TLR-4介导的。这些结果表明，炎症标志物钙卫蛋白在小鼠唾液腺中表达，并且LPS刺激其合成。钙卫蛋白（S100 A8/A9）在SMG的所有细胞节段中显示最小的表达，除了排泄管细胞，其在细胞质中显示强信号。LPS诱导颗粒曲管细胞和纹状管细胞表达。在PG中，这些蛋白在导管和腺泡细胞中表达非常弱，前者细胞的染色略强。LPS注射可诱导导管和腺泡细胞中钙卫蛋白（S100 A8/A9）的表达，尤其是导管和腺泡细胞。
Calprotectin is a major cytosolic calcium-binding protein of leukocytes which belongs to the S100 protein family. S100A8 and S100A9, major types of calprotectin are heterodimeric complexes being composed of light-and heavy-chain subunits. The calprotectin levels in the plasma, feces, synovial fluid, gingival crevicular fluid, dental calculus and saliva change when the host animal suffers from several inflammatory diseases. Members of Toll-like receptor (TLR) family are pattern-recognition receptors for lipopolysaccharide (LPS) and other pathogens. Here we examined if the biological role of TLR receptor is reflected to the calprotectin expression in the salivary gland. Time course study by using real-time RT-PCR detected higher levels of S100A8 and S100A9 mRNA at 1.5-3 h after injection of LPS in both the submandibular gland (SMG) and parotid gland ( PG) of C3H/HeN mice but not in the same tissues of C3H/HeJ, a TLR-4 mutant strain, indicating that this induction is mediated via the TLR-4. These results indicate that, an inflammatory marker, calprotectin, is expressed in the mouse salivary gland and that LPS stimulated its synthesis. Calprotectin (S100A8/A9) showed minimum expression in all cellular segments in the SMG except excretory duct cells, which showed strong signal at the cytoplasm. LPS induced their expressions in the granular convoluted tubular cells and striated duct cells. In the PG, these proteins were expressed very weakly in both duct and acinar cells with a little stronger staining for the former cells. LPS injection induced calprotectin (S100A8/A9) in both duct and acinar cells especially in the former cells.