Chemical imaging and microspectroscopy with spectral focusing coherent anti-Stokes Raman scattering
Chemical imaging and microspectroscopy with spectral focusing coherent anti-Stokes Raman scattering
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DOI：
10.1117/1.3533315
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发表时间：
2011-02-01
影响因子：
3.5
通讯作者：
Lim, Sang-Hyun
中科院分区：
文献类型：
--
作者：
Chen, Bi-Chang;Sung, Jiha;Lim, Sang-Hyun
基于光谱聚焦机制，我们展示了两种不同的相干反斯托克斯拉曼散射(CARS)显微镜和显微光谱方法。第一种方法使用单个钛宝石激光器产生的强啁啾宽带脉冲，并在指纹区域产生CARS信号。泵浦和斯托克斯激光脉冲之间的时间延迟的快速调制与锁定信号检测相结合，显著减少了非共振背景，产生了光谱分辨率为20 cm(-1)的类喇曼CARS信号。第二种方法利用来自光子晶体光纤的红外超连续脉冲在CH(碳氢)伸缩区域产生CARS信号。光谱分辨率达到30 cm(-1)。用最大熵方法从脂膜中提取了拉曼当量CARS光谱。用不同的样品演示了化学成像和显微光谱分析。(C)2011年，光学仪器工程师学会。[DOI：10.1117/1.3533315]
We demonstrate two different coherent anti-Stokes Raman scattering (CARS) microscopy and microspectroscopy methods based on the spectral focusing mechanism. The first method uses strongly chirped broadband pulses from a single Ti: sapphire laser and generates CARS signals at the fingerprint region. Fast modulation of the time delay between the pump and Stokes laser pulses coupled with lock-in signal detection significantly reduces the nonresonant background and produces Raman-like CARS signals with a spectral resolution of 20 cm(-1). The second method generates CARS signals in the CH (carbon-hydrogen) stretching region with IR supercontinuum pulses from a photonic crystal fiber. The spectral resolution of 30 cm(-1) is achieved. Maximum entropy method is used to retrieve a Raman-equivalent CARS spectrum from lipid membranes. Chemical imaging and microspectroscopy are demonstrated with various samples. (C) 2011 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI:10.1117/1.3533315]