Comparative microbial antibiotic resistome between urban and deep forest environments
Comparative microbial antibiotic resistome between urban and deep forest environments
复制标题
城市和深层森林环境之间微生物抗生素耐药性的比较
DOI：
10.1111/1758-2229.12942
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发表时间：
2021
影响因子：
3.3
通讯作者：
Wenjun Jiang
中科院分区：
文献类型：
--
作者：
Yongchang Zheng;Si Yu;Guanqun Wang;Fucun Xie;Haifeng Xu;Shunda Du;Haitao Zhao;Xinting Sang;Jizhou Lu;Wenjun Jiang
使用抗生素根除微生物病原体的一个矛盾的结果是在各种环境中出现大量耐药微生物。环境微生物将不可避免地对几乎每一种临床可用的抗生素产生耐药性的担忧，由于这些耐药基因在不同环境中的传播和多药耐药表型的出现而加剧。在这里，我们提供了对从北京大城市的医院，居民区和森林公园以及云南省深林中收集的16个土壤样品的微生物组和抗性组的宏基因组学见解。使用Illumina HiSeq测序，我们调查了宏基因组鸟枪读数中的微生物多样性，并从这些样本中鉴定出486个抗生素耐药基因（ARG），分为30种类型，其中多药耐药基因最丰富。我们的研究结果提供了一个重要的参考和直接比较的微生物抗生素耐药的土壤样品从一个大城市和深林，扩大我们的了解ARGs在现代城市和自然环境中的传播。
A paradoxical result of using antibiotics to eradicate microbial pathogens is the emergence of a vast number of resistant microbes in various environments. The concern that environmental microbes will inevitably become resistant to virtually every clinically usable antibiotics has been exacerbated by the spread of these resistance genes across different environments and the emergence of multidrug resistant phenotypes. Here, we provide metagenomic insights into the microbiomes and resistomes of 16 soil samples collected from hospitals, residential areas, and forest parks in the megacity of Beijing and deep forests in the Yunnan province. Using Illumina HiSeq sequencing, we investigated the microbial diversity within the metagenomic shotgun reads and identified 486 antibiotic‐resistant genes (ARGs) classified into 30 types from these samples, among which multidrug resistance genes were the most abundant. Our results present an important reference and direct comparison of microbial antibiotic resistomes of soil samples from a megacity and deep forests and extend our understanding of the spread of ARGs in modern urban and natural environments.