Molecular dissection of the migrating posterior lateral line primordium during early development in zebrafish.
Molecular dissection of the migrating posterior lateral line primordium during early development in zebrafish.
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DOI：
10.1186/1471-213x-10-120
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发表时间：
2010-12-13
影响因子：
--
通讯作者：
Burgess SM
中科院分区：
文献类型：
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作者：
Gallardo VE;Liang J;Behra M;Elkahloun A;Villablanca EJ;Russo V;Allende ML;Burgess SM
斑马鱼后侧线系统的发育涉及细胞迁移、增殖和机械感觉细胞的分化。当颅基板细胞分层并成为贯穿鱼的长度以形成该感觉系统的连贯的迁移原基时，PLL形成。当它迁移时，原基沉积了一组称为神经丘的细胞，这是一种包含机械感觉毛细胞的特殊器官。因此，原基为研究集体定向细胞迁移和感觉细胞从多能祖细胞分化提供了模型。通过结合使用转基因鱼，荧光激活细胞分选和微阵列分析，我们确定了一个剧目的迁移原基和分化的神经瘤中表达的关键基因。我们通过定量RT-PCR和原位杂交验证了所鉴定序列的一个子集在原基中的特异性表达。我们还发现，干扰f11 r和cd 9 b两个基因的功能，会导致原基迁移的缺陷。最后，通路的构建揭示了迁移细胞群中富集的基因之间的功能关系。我们的研究结果表明，这是一个强大的方法来全球分析组织特异性表达，我们预测，许多在这项研究中确定的基因将显示在涉及集体细胞迁移的发育事件中的关键功能，并可能在病理情况下，如肿瘤转移。
Development of the posterior lateral line (PLL) system in zebrafish involves cell migration, proliferation and differentiation of mechanosensory cells. The PLL forms when cranial placodal cells delaminate and become a coherent, migratory primordium that traverses the length of the fish to form this sensory system. As it migrates, the primordium deposits groups of cells called neuromasts, the specialized organs that contain the mechanosensory hair cells. Therefore the primordium provides both a model for studying collective directional cell migration and the differentiation of sensory cells from multipotent progenitor cells. Through the combined use of transgenic fish, Fluorescence Activated Cell Sorting and microarray analysis we identified a repertoire of key genes expressed in the migrating primordium and in differentiated neuromasts. We validated the specific expression in the primordium of a subset of the identified sequences by quantitative RT-PCR, and by in situ hybridization. We also show that interfering with the function of two genes, f11r and cd9b, defects in primordium migration are induced. Finally, pathway construction revealed functional relationships among the genes enriched in the migrating cell population. Our results demonstrate that this is a robust approach to globally analyze tissue-specific expression and we predict that many of the genes identified in this study will show critical functions in developmental events involving collective cell migration and possibly in pathological situations such as tumor metastasis.