Stability of metabolites in dried blood spots stored at different temperatures over a 2-year period
Stability of metabolites in dried blood spots stored at different temperatures over a 2-year period
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DOI：
10.4155/bio.13.121
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发表时间：
2013-06-01
期刊：
影响因子：
1.8
通讯作者：
Dalton, R. Neil
中科院分区：
文献类型：
--
作者：
Prentice, Philippa;Turner, Charles;Dalton, R. Neil
背景：定量LC-ESI-MS/MS，从新生儿筛查中发展而来，越来越多地用于靶向代谢物分析。干血斑（DBS）提供了容易获得的生物样品，但长期稳定性数据很少。将DBS储存在环境温度（室温[RT]; 21 ℃）、-20 ℃和-80 ℃下。在12个时间点（0-104周）通过LC-ESI-MS/MS，使用完全定量的稳定同位素稀释法分析代谢物。结果如下：主成分分析表明，在不同的温度下代谢物的稳定性改变，主要变化仅在RT。单变量分析单个分析物的浓度增加或减少。结论：在RT下观察到某些DBS代谢物的显著变化，冷冻时这些代谢物减弱或不存在。这些数据将有助于为未来DBS研究的设计、分析和解释提供信息。
Background: Quantitative LC-ESI-MS/MS, developed from newborn screening, is increasingly used for targeted metabolite profiling. Dried blood spots (DBS) provide easily obtainable biological samples but long-term stability data are sparse. DBS were stored at ambient temperature (room temperature [RT]; 21 degrees C), -20 and -80 degrees C. Metabolites were analyzed at 12 time points (0-104 weeks) by LC-ESI-MS/MS, using fully quantitative stable isotope dilution. Results: Principal component analysis showed alterations in metabolite stability at different temperatures, with major changes only at RT. Univariate analysis for individual analytes demonstrated increases or reductions in concentration. Conclusion: Significant changes are observed in certain DBS metabolites at RT, which are attenuated or not present when frozen. These data will help to inform the design, analysis and interpretation of future DBS studies.