Progression throughout All Stages of Meiosis from the Early Prophase of Newt Primary Spermatocytes in vitro
Progression throughout All Stages of Meiosis from the Early Prophase of Newt Primary Spermatocytes in vitro
复制标题
体外蝾螈原代精母细胞早期减数分裂所有阶段的进展
DOI：
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发表时间：
1983
期刊：
影响因子：
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通讯作者：
Abe Shin
中科院分区：
文献类型：
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作者：
A. Nishikawa;Abe Shin
利用玫瑰培养室测定活原代精母细胞的前期阶段(1)。分离的原代精母细胞在22°C或27°C的胶原基质中以低细胞密度培养，并测量从前期不同阶段到减数分裂到晚期不同阶段的细胞百分比。在标准培养基（Leibovitz‐15 + 10%胎牛血清）中，在22℃下，超过70%的粗线期以上的初级精母细胞可以在几天内发育成带鞭毛的圆形精母细胞。在22°C条件下，至少13%的瘦素细胞在一周内达到中期I。在27°C下进展的细胞百分比略低于22°C：在22°C和27°C下，从瘦素进展到中期I分别需要6.3天和4.3天。胎牛血清对减数分裂的进展并不是必不可少的。此外，0.5-5.0 μg/ml的羊卵泡刺激素（NIAMDD - o - FSH - 13）、0.01-1.0 μg/ml的5α -二氢睾酮和1.0 μg/ml的丙酸睾酮在22℃下对细胞进展率没有显著影响。
Stages of prophase of living primary spermatocytes were determined by use of Rose culture chambers (1). Dissociated primary spermatocytes were cultured at low cell‐density in a collagen matrix at 22°C or 27°C and the percentages of cells which had progressed from various stages in prophase through meiosis to various advanced stages were measured. In a standard medium (Leibovitz‐15 + 10% fetal bovine serum), more than 70% of the primary spermatocytes at stages beyond the pachytene stage could advance to round spermatids with flagella within a few days at 22°C. The percentages of cells that progressed from stages before the late zygotene stage were less, but at least 13 % of leptotene cells reached metaphase I within a week at 22°C. The percentage of cells that progressed was slightly lower at 27°C than at 22°C: 6.3 and 4.3 days were required for progress from leptotene to metaphase I at 22°C and 27C, respectively. Fetal bovine serum was not indispensable for progression through meiosis. Moreover, 0.5–5.0 μg/ml ovine follicle stimulating hormone (NIAMDD‐o‐FSH‐13), 0.01–1.0 μg/ml 5α‐dihydrotestosterone and 1.0 μg/ml testosterone propionate had no significant effect in increasing the percentage of cell progression at 22°C.