Molecular and chemical analysis of β-amylase-less mutant barley in Tibet.
Molecular and chemical analysis of β-amylase-less mutant barley in Tibet.
复制标题
西藏无β-淀粉酶突变大麦的分子和化学分析。
DOI：
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发表时间：
2000
期刊：
影响因子：
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通讯作者：
K. Takeda
中科院分区：
文献类型：
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作者：
T. Kaneko;M. Kihara;K. Ito;K. Takeda
对西藏大麦β-淀粉酶缺失突变体的分子和生化特性进行了研究。酶分析表明，西藏大麦中存在8个β-淀粉酶缺失突变系。经Southern杂交和聚合酶链式反应分析，推测这些突变是由β-淀粉酶结构基因后半部分相同的插入突变所致。突变系萌发初期α-葡萄糖苷酶活性水平较高，萌发种子中糖构象中的葡萄糖比例明显较高。这些结果表明，该β-淀粉酶缺失突变系可能成为啤酒生产的新育种材料。
The molecular and biochemical aspects of Tibetan β-amylase-less mutant barley were investigated. An enzyme assay showed that there were eight β-amylase-less mutant lines in the Tibetan landrace barley. Following Southern analysis and polymerase chain reaction, it is suggested that these mutants result from the same insert mutation in the latter part of the β-amylase structural gene. The α-glucosidase activity level of the mutant line was high in the initial period of germination, and the glucose ratio in the sugar conformation was remarkably high in the germinated seed of the mutant line. These results suggest that this β-amylase-less mutant line could be a new breeding material for beer production.