CLONED AND EXPRESSED NITRIC-OXIDE SYNTHASE STRUCTURALLY RESEMBLES CYTOCHROME-P-450 REDUCTASE
CLONED AND EXPRESSED NITRIC-OXIDE SYNTHASE STRUCTURALLY RESEMBLES CYTOCHROME-P-450 REDUCTASE
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DOI：
10.1038/351714a0
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发表时间：
1991-06-27
期刊：
影响因子：
64.8
通讯作者：
SNYDER, SH
中科院分区：
文献类型：
--
作者：
BREDT, DS;HWANG, PM;SNYDER, SH
一氧化氮是一种信使分子，介导血管内皮源性松弛因子的作用和巨噬细胞的细胞毒性作用，在脑内的神经元通讯中发挥作用。克隆了脑一氧化氮合酶的互补DNA，揭示了NADPH、FAD、黄素单核苷酸和钙调素的识别位点以及磷酸化位点，表明该合酶受多种不同因素的调控。唯一已知的具有密切同源性的哺乳动物酶是细胞色素P-450还原酶。
Nitric oxide is a messenger molecule, mediating the effect of endothelium-derived relaxing factor in blood vessels and the cytotoxic actions of macrophages, and playing a part in neuronal communication in the brain. Cloning of a complementary DNA for brain nitric oxide synthase reveals recognition sites for NADPH, FAD, flavin mononucleotide and calmodulin as well as phosphorylation sites, indicating that the synthase is regulated by many different factors. The only known mammalian enzyme with close homology is cytochrome P-450 reductase.