A Spatial Toggle Switch Drives Boundary Formation in Development
A Spatial Toggle Switch Drives Boundary Formation in Development
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DOI：
10.1529/biophysj.108.133306
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发表时间：
2008-12-01
影响因子：
3.4
通讯作者：
Buceta, Javier
中科院分区：
文献类型：
--
作者：
Canela-Xandri, Oriol;Sagues, Francesc;Buceta, Javier
在这里，我们介绍了一个多细胞网络基序，作为一个空间切换开关，并解释了如何边界的形成可以忠实地完成在发展过程中。重要的是，我们表明，蛋白质的表达和活性模式必须同时进行表征，以正确理解和描述其潜在机制。我们的计算机模拟实验与体内结果一致，评估了不同的遗传背景，并揭示了边界形成的动力学。此外，我们还提供了相关生物学参数的估计和稳健性分析。
Herein we introduce a multicellular network motif that performs as a spatial toggle switch and explains how boundary formation can be faithfully accomplished in developmental processes. Importantly, we show that expression and activity patterns of proteins must be simultaneously characterized for a proper understanding and description of the underlying mechanism. Our in silico experiments, in agreement with in vivo results, evaluate different genetic backgrounds and shed light on the dynamics of boundary formation. In addition, we provide an estimation of relevant biological parameters and a robustness analysis.