Injurious effect of ischemic reperfusion and the protective effect of Yi-Xin-Kang capsule on rat myocardial mitochondria
Injurious effect of ischemic reperfusion and the protective effect of Yi-Xin-Kang capsule on rat myocardial mitochondria
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发表时间：
2001
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通讯作者：
C. Ke
中科院分区：
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作者：
C. Ke
目的观察再灌流对心肌线粒体的损伤作用，评价益心康胶囊(H303)对心肌线粒体的保护作用。方法采用大鼠全心缺血再灌流模型，观察心肌线粒体结构和功能的变化。结果缺血再灌流对心肌线粒体结构和功能造成严重损伤。H303灌流预处理可减轻心肌脂质过氧化反应，减轻心肌细胞的脂质过氧化损伤。H303对心肌线粒体内磷脂酶A2(PLA2)活性、磷脂(PL)和游离脂肪酸(FFA)含量及改善膜脂流动性(LFU)有保护作用，对线粒体呼吸功能有保护作用。结论H303对心肌缺血再灌注损伤具有明显的保护作用。
Objective To observe the injury of mitochondria induced by reperfusion and to evaluate the protective effect of Yi Xin Kang capsule(H3O3),a traditional Chinese medicinal prescription (H303).Method Using the model of global ischemia/reperfusion (I/R) of the rat heart,the changes of the structure and function of myocardial mitochondria were measured.Results The results demonstrated that I/R caused severe injury of the structure and function of myocardial mitochondria.Pretreatment with H303 by perfusion showed protective effects through decreasing lipid peroxidation of myocardium,the activity of phospholipase A 2 (PLA 2) and the contents of phospholipid (PL) and free fat acid (FFA) in myocardial mitochondria and improving membrane lipid fluidity (LFU).In addition,H303 also possessed the effects of protecting the activity of Ca 2+ ATPase and the respiratory function of mitochondria.Conclusion H303 has the significant protective effects on mitochondria injury induced by I/R.