Analysis on DNA sequence of GPR54 gene and its association with litter size in goats
Analysis on DNA sequence of GPR54 gene and its association with litter size in goats
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山羊GPR54基因DNA序列分析及其与产羔数的关系
DOI：
10.1007/s11033-010-0499-y
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发表时间：
2011-08-01
影响因子：
2.8
通讯作者：
Li, N.
中科院分区：
文献类型：
--
作者：
Cao, G. L.;Chu, M. X.;Li, N.
Kispeptin/GPr54通路在青春期启动过程中起着至关重要的作用。设计6对引物克隆山羊GPR54基因，对GPR54基因的多态性进行扫描，并用1对引物检测GPR54基因在性早熟和有性晚熟山羊品种中的多态性。获得了山羊GPR54的DNA片段，全长4258bp，包含1137bp的开放阅读框(ORF)，编码378个氨基酸，与其他哺乳动物有很高的同源性。该蛋白被预测有七个跨膜区。测序结果表明，山羊外显子1~4无碱基变异，外显子5有3个碱基突变(G4014A、G4136A和C4152T)，这3个突变可能与山羊性早熟有关。4152位点济宁青山羊TT和CT基因座分别比CC基因座多产仔1.02头和0.84头(P<0.01)。济宁青山羊TT型和CT型间产羔数差异不显著(P>；0.05)。济宁青山羊不同基因型间产羔数差异不显著(P>；0.05)。本研究初步揭示了GPR54基因4152位点的T等位基因与济宁青山羊产羔数的相关性。
The kisspeptin/GPR54 pathway is crucial in the process of puberty onset. Six pairs of primers were designed to clone goat GPR54 and scan polymorphisms and one pair of primers to detect polymorphisms of GPR54 in sexual precocious and sexual late-maturing goat breeds. A DNA fragment of 4258 bp of goat GPR54 was obtained, which contains an open reading frame (ORF) of 1137 bp and encodes 378 amino acids, having a high homology with other mammals. The protein was predicted to have seven transmembrane regions. There were no base pair variation in exons 1-4 and three base changes (G4014A, G4136A and C4152T) in exon 5 by sequencing and the three mutations may have some correlation with sexual precocity in goats. For the 4152 locus, the Jining Grey goat does with genotype TT and CT had 1.02 and 0.84 (P<0.01) kids more than those with genotype CC, respectively. No significant difference (P>0.05) was found in litter size between TT and CT genotypes in Jining Grey goat. For the other two loci, no significant difference (P>0.05) was found in litter size between different genotypes in Jining Grey goats. The present study preliminarily indicated an association between allele T of the 4152 locus in GPR54 and high litter size in Jining Grey goats.