A loss of profilin-1 in late-stage oral squamous cell carcinoma.
A loss of profilin-1 in late-stage oral squamous cell carcinoma.
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DOI：
10.1111/jop.12523
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发表时间：
2017-08
期刊：
影响因子：
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通讯作者：
Schwartz JL
中科院分区：
文献类型：
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作者：
Adami GR;O'Callaghan TN;Kolokythas A;Cabay RJ;Zhou Y;Schwartz JL
PFN1和TMSB4基因都在口腔组织中高度表达，都编码肌动蛋白单体结合蛋白，被认为在细胞运动和可能是肿瘤进展的其他关键部分发挥作用。采用口腔刷状细胞学方法检测口腔鳞癌组织中PFN1和TMSB4mRNA的表达，并用免疫组织化学方法检测组织中的蛋白水平。我们观察到编码这两种蛋白的mRNAs的高度但可变的表达，这表明它们可能与口腔鳞状细胞癌的亚群中的肿瘤特征有关。这两种蛋白在正常的基底上皮、胞浆和核周均有高表达，而在上皮层表达极少。在口腔鳞状细胞癌中，这些蛋白的表达各不相同。在晚期肿瘤中，根据肿瘤的大小和/或淋巴转移，肿瘤上皮中的PFN1水平较低。对照基因KRT13在正常分化的基底层和基底层以上口腔黏膜上皮细胞中均有表达，而在口腔鳞癌细胞中缺失。在其他类型的肿瘤中，肿瘤细胞中PFN1的缺失与淋巴侵袭和转移有关，这加强了该蛋白在晚期口腔鳞癌中具有潜在的肿瘤抑制作用的论点。
The genes for PFN1 and TMSB4 are both highly expressed in oral tissue and both encode actin monomer binding proteins thought to play a role in cell motility and possibly other crucial parts of tumor progression. Oral brush cytology of epithelium from Oral Squamous Cell Carcinoma (OSCC) was used to measure PFN1 and TMSB4 mRNA in OSCC while immunohistochemical analysis of tissue was used to check protein levels. High but variable expression of mRNAs encoding these two proteins was observed suggesting they may contribute to tumor characteristics in a subset of OSCCs. Both proteins were highly expressed in normal appearing basal epithelium, in the cytoplasm and perinuclear area, while expression was minimal in upper epithelial layers. In OSCCs, expression of these proteins varied. In tumors classified as later stage, based on size and/or lymph node involvement, PFN1 levels were lower in tumor epithelium. A control gene, KRT13, showed expression in normal differentiated basal and suprabasal oral mucosa epithelial cells and as reported was lost in OSCC cells. Loss of PFN1in tumor cells has been associated with lymph node invasion and metastasis in other tumor types, strengthening the argument that the protein has the potential to be a tumor suppressor in late stage OSCC.