Effects of FHL1 and P21 on hypoxia-induced pulmonary vascular remodeling in neonatal rats.
Effects of FHL1 and P21 on hypoxia-induced pulmonary vascular remodeling in neonatal rats.
复制标题
FHL1 和 P21 对缺氧诱导的新生大鼠肺血管重塑的影响。
DOI：
10.3892/etm.2017.5055
复制
发表时间：
2017-11
影响因子：
2.7
通讯作者：
Xue X
中科院分区：
文献类型：
--
作者：
Du Y;Fu J;Yao L;Zhang D;Liu N;Xue X
Numerous studies have demonstrated that altered expression levels of four and a half LIM domains 1 (FHL1) and P21 are necessary for hypoxia-induced pulmonary vascular remodeling in both adult rats and human patients with idiopathic pulmonary arterial hypertension. However, whether FHL1 and P21 are present in the pulmonary artery and whether these proteins affect pulmonary vascular remodeling in hypoxia-induced pulmonary hypertension (HPH) in neonatal rats remain unknown. The present study investigated the effects of altered FHL1 and P21 expression on pulmonary vascular remodeling in neonatal rats with HPH. A total of 32 newborn Sprague-Dawley rats were exposed to hypoxia or room air for 7 or 14 days (n=8/subgroup). Parameters including the percentage of medial wall thickness (WT%), the percentage of medial wall area (WA%), right ventricular (RV) mean pressure, RV hypertrophy index (RVHI) and RV systolic pressure (RVSP) were measured to evaluate the development of HPH. Additionally, the expressions of FHL1 and P21 in the pulmonary artery smooth muscle cells (PASMCs) were measured by reverse transcription-quantitative polymerase chain reaction, western blot analysis and immunohistochemical staining. WA%, WT%, RV mean pressure, RVHI and RVSP were significantly increased in the HPH model group when compared with the control group (P<0.01). The protein expression levels of FHL1 were significantly increased in the HPH group (P<0.05), while the mRNA and protein expression levels of P21 were significantly reduced (P<0.05). Pearson correlation analysis indicated that the protein expressions of FHL1 and P21 were correlated with WA% and WT% (all P<0.001), and that the protein expression of P21 was negatively correlated with that of FHL1 (P<0.01). The results indicated that the expressions of FHL1 and P21 were altered in the PASMCs of newborn rats with HPH. Furthermore, FHL1 and P21 may serve important roles in pulmonary vascular remodeling.
登录
查看更多内容
低氧诱导因子信号传导和新生儿的实验持续性肺动脉高压。
影响因子：
5.6
作者：
Wedgwood S;Lakshminrusimha S;Schumacker PT;Steinhorn RH
通讯作者：
Steinhorn RH
影响因子：
2.5
作者：
Huetsch JC;Jiang H;Larrain C;Shimoda LA
通讯作者：
Shimoda LA
影响因子：
33.5
作者：
Xiang H;Yuan L;Gao X;Alexander PB;Lopez O;Lau C;Ding Y;Chong M;Sun T;Chen R;Liu SQ;Wu H;Wan Y;Randell SH;Li QJ;Wang XF
通讯作者：
Wang XF
FHL 家族成员通过阻断 HIF1α 和 HIF1β 二聚化抑制血管内皮生长因子表达
影响因子：
4.6
作者：
Lin, Jing;Qin, Xi;Ye, Qinong
通讯作者：
Ye, Qinong
影响因子：
3.6
作者：
Nadeau, S;Baribeau, J;Perreault, T
通讯作者：
Perreault, T